Virus spread, tissue inflammation and antiviral response in brains of flavivirus susceptible and resistant mice acutely infected with Murray Valley encephalitis virus

Silvia, O. J., Pantelic, L., Mackenzie, J. S., Shellam, G. R., Papadimitriou, J. and Urosevic, N. (2004) Virus spread, tissue inflammation and antiviral response in brains of flavivirus susceptible and resistant mice acutely infected with Murray Valley encephalitis virus. Archives of Virology, 149 3: 447-464. doi:10.1007/s00705-003-0238-y


Author Silvia, O. J.
Pantelic, L.
Mackenzie, J. S.
Shellam, G. R.
Papadimitriou, J.
Urosevic, N.
Title Virus spread, tissue inflammation and antiviral response in brains of flavivirus susceptible and resistant mice acutely infected with Murray Valley encephalitis virus
Formatted title
Virus spread, tissue inflammation and antiviral response in brains of flavivirus susceptible and resistant mice acutely infected with Murray Valley encephalitis virus
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
1432-8798
0939-1983
Publication date 2004-03
Year available 2003
Sub-type Article (original research)
DOI 10.1007/s00705-003-0238-y
Volume 149
Issue 3
Start page 447
End page 464
Total pages 18
Place of publication Wien, Austria
Publisher Springer
Collection year 2004
Language eng
Subject C1
270303 Virology
780105 Biological sciences
0605 Microbiology
Formatted abstract
Inborn resistance to flaviviruses, conferred by a single chromosome 5 locus Flv, is a genetic trait operative in wild mice and a few strains of laboratory mice. In this study we have used in situ hybridisation to trace the spread of flavivirus genomic RNA within the brains of flavivirus susceptible C3H/HeJARC and congenic resistant C3H.PRI-Flvr mice following infection with Murray Valley encephalitis virus (MVE) in parallel to studying a brain histopathology and induction of cellular genes involved in antiviral response. We find that in contrast to a high viral RNA content in brains of susceptible mice, viral RNA was markedly reduced in the cortex, olfactory bulb, thalamus and hypothalamus of resistant mice. Trace amounts of viral RNA were detected in the medulla oblongata while it was completely absent from the hippocampus, pons and cerebellum of resistant mice at different time points post infection. The low virus titres within brains of resistant mice coincided with a very mild inflammation, low counts of infiltrating inflammatory cells, and lower IFN I/II and TNFα gene induction than in susceptible mice. Furthermore, transcripts of several genes belonging to a 2′,5′-oligoadenylate synthetase (OAS) family, implicated in IFN I-inducible OAS/RNase L antiviral pathway, showed similar brain tissue induction in both strains of mice suggesting only minor contribution of this pathway to the resistance phenotype.
© Springer-Verlag 2003
Keyword Virology
West-nile-virus
Innate resistance
Nitric-oxide
Mouse chromosome-5
Locus Flv
Gene
Interferon
Synthetase
Strains
Arboviruses
Q-Index Code C1
Additional Notes Published online: 13 November 2003

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Science Publications
Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 04:54:54 EST