Differential effects on the hepatitis C virus (HCV) internal ribosome entry site by vitamin B-12 and the HCV core protein

Li, Dongsheng, Lott, William B., Martyn, John, Haqshenas, Gholamreza and Gowans, Eric J. (2004) Differential effects on the hepatitis C virus (HCV) internal ribosome entry site by vitamin B-12 and the HCV core protein. Journal of Virology, 78 21: 12075-12081. doi:10.1128/JVI.78.21.12075-12081.2004


Author Li, Dongsheng
Lott, William B.
Martyn, John
Haqshenas, Gholamreza
Gowans, Eric J.
Title Differential effects on the hepatitis C virus (HCV) internal ribosome entry site by vitamin B-12 and the HCV core protein
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2004-11
Year available 2004
Sub-type Article (original research)
DOI 10.1128/JVI.78.21.12075-12081.2004
Open Access Status DOI
Volume 78
Issue 21
Start page 12075
End page 12081
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2004
Language eng
Subject C1
270303 Virology
780105 Biological sciences
Abstract To investigate the role of the hepatitis C virus internal ribosome entry site (HCV IRES) domain IV in translation initiation and regulation, two chimeric IRES elements were constructed to contain the reciprocal domain IV in the otherwise HCV and classical swine fever virus IRES elements. This permitted an examination of the role of domain IV in the control of HCV translation. A specific inhibitor of the HCV IRES, vitamin B-12 was shown to inhibit translation directed by all IRES elements which contained domain IV from the HCV and the GB virus B IRES elements, whereas the HCV core protein could only suppress translation from the wild-type HCV IRES. Thus, the mechanisms of translation inhibition by vitamin B-12 and the core protein differ, and they target different regions of the IRES.
Keyword Virology
Swine-fever Virus
5 Noncoding Region
Mediated Translation
Dependent Translation
Coding Sequences
Initiation Codon
Rna Pseudoknot
Replication
Binding
Inhibition
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 04:54:33 EST