Cortactin is necessary for E-cadherin-mediated contact formation and actin reorganization

Helwani, F. M., Kovacs, E. M., Paterson, A. D., Verma, S., Ali, R. G., Fanning, A. S., Weed, S. A. and Yap, A. S. (2004) Cortactin is necessary for E-cadherin-mediated contact formation and actin reorganization. Journal of Cell Biology, 164 6: 899-910. doi:10.1083/jcb.200309034

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Author Helwani, F. M.
Kovacs, E. M.
Paterson, A. D.
Verma, S.
Ali, R. G.
Fanning, A. S.
Weed, S. A.
Yap, A. S.
Title Cortactin is necessary for E-cadherin-mediated contact formation and actin reorganization
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
Publication date 2004-03-15
Sub-type Article (original research)
DOI 10.1083/jcb.200309034
Open Access Status File (Publisher version)
Volume 164
Issue 6
Start page 899
End page 910
Total pages 12
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Collection year 2004
Language eng
Formatted abstract
Classical cadherin adhesion molecules are key determinants of cell-cell recognition during development and in post-embryonic life. A decisive step in productive cadherin-based recognition is the conversion of nascent adhesions into stable zones of contact. It is increasingly clear that such contact zone extension entails active cooperation between cadherin adhesion and the force-generating capacity of the actin cytoskeleton. Cortactin has recently emerged as an important regulator of actin dynamics in several forms of cell motility. We now report that cortactin is recruited to cell-cell adhesive contacts in response to homophilic cadherin ligation. Notably, cortactin accumulates preferentially, with Arp2/3, at cell margins where adhesive contacts are being extended. Recruitment of cortactin is accompanied by a ligation-dependent biochemical interaction between cortactin and the cadherin adhesive complex. Inhibition of cortactin activity in cells blocked Arp2/3-dependent actin assembly at cadherin adhesive contacts, significantly reduced cadherin adhesive contact zone extension, and perturbed both cell morphology and junctional accumulation of cadherins in polarized epithelia. Together, our findings identify a necessary role for cortactin in the cadherin-actin cooperation that supports productive contact formation.
© The Rockefeller University Press
Keyword Cell Biology
Cortactin actin assembly
Cell-cell adhesion
Arp2/3 complex
Phosphatidylinositol 3-kinase
Tyrosine phosphorylation
Membrane protrusion
Q-Index Code C1

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Citation counts: TR Web of Science Citation Count  Cited 124 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 04:50:47 EST