Acute cadmium chloride administration induces hepatic and renal cytochrome P450 2A5 in the mouse

Moore, Michael R., Abu-Bakar, A'edah, Satarug, Soisungwan, Marks, Geoffrey C. and Lang, Matti A. (2004). Acute cadmium chloride administration induces hepatic and renal cytochrome P450 2A5 in the mouse. In: Toxicology and Applied Pharmacology. Living in a Safe Chemical World: ICTX 2004, Tampere, Finland, (272-273). 11 - 15 July, 2004.


Author Moore, Michael R.
Abu-Bakar, A'edah
Satarug, Soisungwan
Marks, Geoffrey C.
Lang, Matti A.
Title of paper Acute cadmium chloride administration induces hepatic and renal cytochrome P450 2A5 in the mouse
Conference name Living in a Safe Chemical World: ICTX 2004
Conference location Tampere, Finland
Conference dates 11 - 15 July, 2004
Proceedings title Toxicology and Applied Pharmacology   Check publisher's open access policy
Journal name Toxicology and Applied Pharmacology   Check publisher's open access policy
Place of Publication San Diego, USA
Publisher Academic Press
Publication Year 2004
DOI 10.1016/j.taap.2004.04.002
ISSN 0041-008X
1096-0333
Volume 197
Issue 3
Start page 272
End page 273
Total pages 2
Language eng
Abstract/Summary Modulation of the cytochrome P450 (CYP) monooxygenase system by cadmium was investigated in male, adult DBA/2J mice treated with a single dose (16 Amol/kg body weight, i.p.) of cadmium chloride (CdCl2) at various time points. The total CYP content of kidney microsomes started to decrease 4 hours earlier than in the liver (P < 0.05), with maximal decreases at 24 hours of 56% and 85% in the liver and kidney, respectively. In contrast, both hepatic and renal coumarin 7-hydroxylase (COH) activity (indicative of CYP2A5 activity) relative to total CYP content started to progressively increase at 8 hours, with renal activity 61 times higher than the hepatic activity. Maximum increases were observed, 15-fold in the liver and 64-fold in the kidney after 24 hours. Liver and kidney CYP2A5 mRNA levels increased maximally 12 and 4 hours after treatment, respectively and decreased to almost half 6 hours later. In contrast, kidney and liver CYP2A5 protein levels increased maximally at 18 and 24 hours. This study demonstrates that hepatic and renal CYP2A5 is upregulated by cadmium with a faster response in the kidney than in the liver. This observation is concordant with the fact that kidney is the target organ for cadmium toxicity. The observed increase in the mRNA but not in protein levels after maximal induction suggests involvement of post-transcriptional mechanisms in the regulation of CYP2A5 expression by cadmium.
Subjects CX
321299 Public Health and Health Services not elsewhere classified
730210 Environmental health
0502 Environmental Science and Management
1117 Public Health and Health Services
Keyword Pharmacology & Pharmacy
Toxicology
Cadmium chloride
Cytochrome P450 2A5
Q-Index Code CX

 
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Created: Wed, 15 Aug 2007, 04:49:50 EST