Oxygen-regulated gene expression in bovine blastocysts

Harvey, A. J., Kind, K. L., Pantaleon, M., Armstrong, D. T. and Thompson, J. G. (2004) Oxygen-regulated gene expression in bovine blastocysts. Biology of Reproduction, 71 4: 1108-1119. doi:10.1095/biolreprod.104.028639

Author Harvey, A. J.
Kind, K. L.
Pantaleon, M.
Armstrong, D. T.
Thompson, J. G.
Title Oxygen-regulated gene expression in bovine blastocysts
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 2004
Sub-type Article (original research)
DOI 10.1095/biolreprod.104.028639
Volume 71
Issue 4
Start page 1108
End page 1119
Total pages 12
Editor J. J. Eppig
M. A. Handel et al.
Place of publication Madison, Wisconsin, USA
Publisher Society for the Study of Reproduction, Inc.
Collection year 2004
Language eng
Subject C1
321026 Reproduction
730116 Reproductive system and disorders
Abstract Oxygen concentrations used during in vitro embryo culture can influence embryo development, cell numbers, and gene expression. Here we propose that the preimplantation bovine embryo possesses a molecular mechanism for the detection of, and response to, oxygen, mediated by a family of basic helix-loop-helix transcription factors, the hypoxia-inducible factors (HIFs). Day 5 compacting bovine embryos were cultured under different oxygen tensions (2%, 7%, 20%) and the effect on the expression of oxygen-regulated genes, development, and cell number allocation and HIFalpha protein localization were examined. Bovine in vitro-produced embryos responded to variations in oxygen concentration by altering gene expression. GLUT1 expression was higher following 2% oxygen culture compared with 7% and 20% cultured blastocysts. HIF mRNA expression (HIF1alpha, HIF2alpha) was unaltered by oxygen concentration. HIF2alpha protein was predominantly localized to the nucleus of blastocysts. In contrast, HIF1alpha protein was undetectable at any oxygen concentration or in the presence of the HIF protein stabilizer desferrioxamine (DFO), despite being detectable in cumulus cells following normal maturation conditions, acute anoxic culture, or in the presence of DFO. Oxygen concentration also significantly altered inner cell mass cell proportions at the blastocyst stage. These results suggest that oxygen can influence gene expression in the bovine embryo during postcompaction development and that these effects may be mediated by HIF2alpha.
Keyword Reproductive Biology
Developmental Biology
Early Development
Gene Regulation
Hypoxia-inducible Factor-1
Developmentally Important Genes
Human Preimplantation Embryos
Factor Messenger-rna
In-vitro Culture
Transcriptional Regulation
Splice Variants
Sheep Conceptus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 94 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 105 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 04:46:38 EST