Dynamic and regulated association of caveolin with lipid bodies: Modulation of lipid body motility and function by a dominant negative mutant

Pol, Albert, Martin, Sally, Fernandez, Manuel A., Ferguson, Charles, Carozzi, Amanda, Luetterforst, Robert, Enrich, Carlos and Parton, Robert G. (2004) Dynamic and regulated association of caveolin with lipid bodies: Modulation of lipid body motility and function by a dominant negative mutant. Molecular Biology of The Cell, 15 1: 99-110. doi:10.1091/mbc.E03-06-0368

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Author Pol, Albert
Martin, Sally
Fernandez, Manuel A.
Ferguson, Charles
Carozzi, Amanda
Luetterforst, Robert
Enrich, Carlos
Parton, Robert G.
Title Dynamic and regulated association of caveolin with lipid bodies: Modulation of lipid body motility and function by a dominant negative mutant
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2004-01
Year available 2003
Sub-type Article (original research)
DOI 10.1091/mbc.E03-06-0368
Open Access Status File (Publisher version)
Volume 15
Issue 1
Start page 99
End page 110
Total pages 12
Place of publication Bethesda
Publisher American Society for Cell Biology
Collection year 2004
Language eng
Subject C1
270104 Membrane Biology
780106 Political science and public policy
Abstract Caveolins are a crucial component of caveolae but have also been localized to the Golgi complex, and, under some experimental conditions, to lipid bodies (LBs). The physiological relevance and dynamics of LB association remain unclear. We now show that endogenous caveolin-1 and caveolin-2 redistribute to LBs in lipid loaded A431 and FRT cells. Association with LBs is regulated and reversible; removal of fatty acids causes caveolin to rapidly leave the lipid body. We also show by subcellular fractionation, light and electron microscopy that during the first hours of liver regeneration, caveolins show a dramatic redistribution from the cell surface to the newly formed LBs. At later stages of the regeneration process (when LBs are still abundant), the levels of caveolins in LBs decrease dramatically. As a model system to study association of caveolins with LBs we have used brefeldin A (BFA). BFA causes rapid redistribution of endogenous caveolins to LBs and this association was reversed upon BFA washout. Finally, we have used a dominant negative LB-associated caveolin mutant (cav(DGV)) to study LB formation and to examine its effect on LB function. We now show that the cav(DGV) mutant inhibits microtubule-dependent LB motility and blocks the reversal of lipid accumulation in LBs.
Keyword Cell Biology
Fatty-acid Composition
Storage Droplets
Rat-liver
Cholesterol
Protein
Cells
Surface
Complex
Domain
Identification
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 04:36:53 EST