Insulin acts via mitogen-activated protein kinase phosphorylation in rabbit blastocysts

Santos, Anne Navarrete, Tonack, Sarah, Kirstein, Michaela, Pantaleon, Marie, Kaye,Peter and Fischer,Bernd (2004) Insulin acts via mitogen-activated protein kinase phosphorylation in rabbit blastocysts. Reproduction, 128 5: 517-526. doi:10.1530/rep.1.00204

Author Santos, Anne Navarrete
Tonack, Sarah
Kirstein, Michaela
Pantaleon, Marie
Title Insulin acts via mitogen-activated protein kinase phosphorylation in rabbit blastocysts
Journal name Reproduction   Check publisher's open access policy
ISSN 1470-1626
Publication date 2004
Sub-type Article (original research)
DOI 10.1530/rep.1.00204
Volume 128
Issue 5
Start page 517
End page 526
Total pages 10
Editor J. Carroll
Place of publication Bristol, UK
Publisher Society for Reproduction and Fertility
Collection year 2004
Language eng
Subject C1
321026 Reproduction
780105 Biological sciences
Abstract The addition of insulin during in vitro culture has beneficial effects on rabbit preimplantation embryos leading to increased cell proliferation and reduced apoptosis. We have previously described the expression of the insulin receptor (IR) and the insulin-responsive glucose transporters (GLUT) 4 and 8 in rabbit preimplantation embryos. However, the effects of insulin on IR signaling and glucose metabolism have not been investigated in rabbit embryos. In the present study, the effects of 170 nM insulin on IR, GLUT4 and GLUT8 mRNA levels, Akt and Erk phosphorylation, GLUT4 translocation and methyl glucose transport were studied in cultured day 3 to day 6 rabbit embryos. Insulin stimulated phosphorylation of the mitogen-activated protein kinase (MAPK) Erk1/2 and levels of IR and GLUT4 mRNA, but not phosphorylation of the phosphatidylinositol 3-kinase-dependent protein kinase, Akt, GLUT8 mRNA levels, glucose uptake or GLUT4 translocation. Activation of the MAPK signaling pathway in the absence of GLUT4 translocation and of a glucose transport response suggest that in the rabbit preimplantation embryo insulin is acting as a growth factor rather than a component of glucose homeostatic control.
Keyword Developmental Biology
Reproductive Biology
Glucose-transporter Expression
Mouse Preimplantation Development
Stimulated Glut4 Translocation
3t3-l1 Adipocytes
Bovine Embryos
Glucose-transporter-4 Translocation
Phosphatidylinositol 3-kinase
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 04:32:37 EST