Identification of the facilitative glucose transporter 12 gene Glut12 in mouse preimplantation embryos

Zhou, Yuchan, Kaye, Peter L. and Pantaleon, Marie (2004) Identification of the facilitative glucose transporter 12 gene Glut12 in mouse preimplantation embryos. Gene Expression Patterns, 4 6: 621-631. doi:10.1016/j.modgep.2004.04.010


Author Zhou, Yuchan
Kaye, Peter L.
Pantaleon, Marie
Title Identification of the facilitative glucose transporter 12 gene Glut12 in mouse preimplantation embryos
Formatted title
Identification of the facilitative glucose transporter 12 gene Glut12 in mouse preimplantation embryos
Journal name Gene Expression Patterns   Check publisher's open access policy
ISSN 1567-133X
1872-7298
Publication date 2004-10-01
Sub-type Article (original research)
DOI 10.1016/j.modgep.2004.04.010
Volume 4
Issue 6
Start page 621
End page 631
Total pages 11
Place of publication Amsterdam. The Netherlands
Publisher Elsevier
Collection year 2004
Language eng
Subject C1
321026 Reproduction
780105 Biological sciences
0604 Genetics
Formatted abstract
In this study we report the cloning and characterisation of the mouse Glut12 gene and examine for the first time its expression pattern in the earliest stages of development. Mouse Glut12 (mGlut12) was cloned from preimplantation embryos by 5'RACE RT-PCR using primers designed from an EST clone corresponding to a human GLUT12 antigenic sequence after positive immunoreactivity was observed in mouse two-cell embryos by western immunoblotting. The mGlut12 gene contains an open reading frame of 1869 base pairs, potentially encoding a polypeptide of 622 amino acids. The predicted mGLUT12 protein bears all the hallmarks of the SLC2A family of hexose transporters and shares an 83% sequence homology to human GLUT12. Consistent with its human homolog mGlut12 mRNA is found highly expressed in skeletal and cardiac muscle and fat. Additionally, it was also found in the uterus and during early embryogenesis. During early development in the mouse, Glut12 expression is clearly apparent in ovulated oocytes and two-cell embryos but declines in day 3 morulae. With the exception of some Glut12 expression apparent in blastocysts, Glut12 mRNA remains at low to undetectable levels until E11.
Keyword Developmental Biology
Genetics & Heredity
Glut12
Preimplantation Embryo
2-cell
Glucose Transport
Blastocyst
Elegans Embryos
Expression
Embryogenesis
Oocytes
Oct-4
Skn-1
Cells
Fate
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 14:31:30 EST