Metamorphosis of a scleractinian coral in response to microbial biofilms

Webster, NS, Smith, LD, Heyward, AJ, Watts, JEM, Webb, RI, Blackall, LL and Negri, AP (2004) Metamorphosis of a scleractinian coral in response to microbial biofilms. Applied And Environmental Microbiology, 70 2: 1213-1221. doi:10.1128/AEM.70.2.1213-1221.2004

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Author Webster, NS
Smith, LD
Heyward, AJ
Watts, JEM
Webb, RI
Blackall, LL
Negri, AP
Title Metamorphosis of a scleractinian coral in response to microbial biofilms
Journal name Applied And Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2004
Sub-type Article (original research)
DOI 10.1128/AEM.70.2.1213-1221.2004
Open Access Status File (Publisher version)
Volume 70
Issue 2
Start page 1213
End page 1221
Total pages 9
Editor Judy D
Wall
L. Nicholas Ornston
Place of publication United States
Publisher American Society for Microbiology
Collection year 2004
Language eng
Subject C1
270399 Microbiology not elsewhere classified
779999 Other
780105 Biological sciences
270800 Biotechnology
Abstract Microorganisms have been reported to induce settlement and metamorphosis in a wide range of marine invertebrate species. However, the primary cue reported for metamorphosis of coral larvae is calcareous coralline algae (CCA). Herein we report the community structure of developing coral reef biofilms and the potential role they play in triggering the metamorphosis of a scleractinian coral. Two-week-old biofilms induced metamorphosis in less than 10% of larvae, whereas metamorphosis increased significantly on older biofilms, with a maximum of 41% occurring on 8-week-old microbial films. There was a significant influence of depth in 4- and 8-week biofilms, with greater levels of metamorphosis occurring in response to shallow-water communities. Importantly, larvae were found to settle and metamorphose in response to microbial biofilms lacking CCA from both shallow and deep treatments, indicating that microorganisms not associated with CCA may play a significant role in coral metamorphosis. A polyphasic approach consisting of scanning electron microscopy, fluorescence in situ hybridization (FISH), and denaturing gradient gel electrophoresis (DGGE) revealed that coral reef biofilms were comprised of complex bacterial and microalgal communities which were distinct at each depth and time. Principal-component analysis of FISH data showed that the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Cytophaga-Flavobacterium of Bacteroidetes had the largest influence on overall community composition. A low abundance of Archaea was detected in almost all biofilms, providing the first report of Archaea associated with coral reef biofilms. No differences in the relative densities of each subdivision of Proteobacteria were observed between slides that induced larval metamorphosis and those that did not. Comparative cluster analysis of bacterial DGGE patterns also revealed that there were clear age and depth distinctions in biofilm community structure; however, no difference was detected in banding profiles between biofilms which induced larval metamorphosis and those where no metamorphosis occurred. This investigation demonstrates that complex microbial communities can induce coral metamorphosis in the absence of CCA.
Keyword Microbiology
Polychaete Hydroids-elegans
Marine Invertebrate Larvae
Gram-positive Bacteria
In-situ Detection
Dna G+c Content
Tasmanian Waters
Settlement
Algae
Induction
Recruitment
Biotechnology & Applied Microbiology
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 04:19:44 EST