tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes

Gu, Wenyi, Li, Mengrong, Zhao, Wei Ming, Fang, Ning Xia, Bu, Shurui, Frazer, Ian H. and Zhao, Kong-Nan (2004) tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes. Nucleic Acids Research, 32 15: 4448-4461. doi:10.1093/nar/gkh748

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Author Gu, Wenyi
Li, Mengrong
Zhao, Wei Ming
Fang, Ning Xia
Bu, Shurui
Frazer, Ian H.
Zhao, Kong-Nan
Title tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes
Journal name Nucleic Acids Research   Check publisher's open access policy
ISSN 0305-1048
1362-4962
Publication date 2004
Sub-type Article (original research)
DOI 10.1093/nar/gkh748
Open Access Status DOI
Volume 32
Issue 15
Start page 4448
End page 4461
Total pages 14
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2004
Language eng
Abstract Exogenous transfer RNAs (tRNAs) favor translation of bovine papillomavirus 1 wild-type (wt) L1 mRNA in in vitro translation systems (Zhou et al. 1999, J. Virol., 73, 4972-4982). We, therefore, investigated whether papillomavirus (PV) wt L1 protein expression could be enhanced in eukaryotic cells following exogenous tRNA supplementation. Both Chinese hamster ovary (CHO) and Cos1 cells, transfected with PV1 wt L1 genes, effectively transcribed the genes but did not translate them. However, L1 protein translation was demonstrated following co-transfection with the L1 gene and a gene expressing tRNA(Ser)(CGA). Cell lines, stably transfected with a bovine papillomavirus 1 (BPV1) wt L1 expression construct, produced L1 protein after the transfection of the tRNA(Ser)(CGA) gene, but not following the transfection with basal vectors, suggesting that tRNA(Ser)(CGA) gene enhanced wt L1 translation as a result of endogenous tRNA alterations and phosphorylation of translation initiation factors elF4E and elF2alpha in the tRNA(Ser)(CGA) transfected L1 cell lines. The tRNA(Ser)(CGA) gene expression significantly reduced translation of L1 proteins expressed from codon-modified (HB) PV L1 genes utilizing mammalian preferred codons, but had variable effects on translation of green fluorescent proteins (GFPs) expressed from six serine GFP variants. The changes of tRNA pools appear to match the codon composition of PV wt and HB L1 genes and serine GFP variants to regulate translation of their mRNAs. These findings demonstrate for the first time in eukaryotic cells that translation of the target genes can be differentially influenced by the provision of a single tRNA expression construct.
Keyword Human-immunodeficiency-virus
Escherichia-coli
Saccharomyces-cerevisiae
Bovine papillomavirus
Mammalian-cells
Transfer-rnas
Protein-synthesis
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 04:09:09 EST