The critical role of tryptophan-116 in the catalytic cycle of dimethylsulfoxide reductase from Rhodobacter capsulatus

Ridge, J. P., Aguey-Zinsou, K. F., Bernhardt, P. V., Hanson, G. R. and McEwan, A. G. (2004) The critical role of tryptophan-116 in the catalytic cycle of dimethylsulfoxide reductase from Rhodobacter capsulatus. FEBS Letters, 563 1-3: 197-202. doi:10.1016/S0014-5793(04)00301-1


Author Ridge, J. P.
Aguey-Zinsou, K. F.
Bernhardt, P. V.
Hanson, G. R.
McEwan, A. G.
Title The critical role of tryptophan-116 in the catalytic cycle of dimethylsulfoxide reductase from Rhodobacter capsulatus
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 2004
Sub-type Article (original research)
DOI 10.1016/S0014-5793(04)00301-1
Volume 563
Issue 1-3
Start page 197
End page 202
Total pages 6
Place of publication Amsterdam
Publisher Elsevier Science Bv
Collection year 2004
Language eng
Subject C1
270108 Enzymes
780105 Biological sciences
Abstract In dimethylsulfoxide reductase of Rhodobacter capsulatus tryptophan-116 forms a hydrogen bond with a single oxo ligand bound to the molybdenum ion. Mutation of this residue to phenylalanine affected the UV/visible spectrum of the purified Mo-VI form of dimethylsulfoxide reductase resulting in the loss of the characteristic transition at 720 nm. Results of steady-state kinetic analysis and electrochemical studies suggest that tryptophan 116 plays a critical role in stabilizing the hexacoordinate monooxo Mo-VI form of the enzyme and prevents the formation of a dioxo pentacoordinate Mo-VI species, generated as a consequence of the dissociation of one of the dithiolene ligands of the molybdopterin cofactor from the Mo ion. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Keyword Biophysics
Cell Biology
Dimethylsulfoxide Reductase
Molybdenum Enzyme
Site-directed Mutagenesis
Electrochemistry
Rhodobacter Capsulatus
Dimethyl-sulfoxide Reductase
Crystal-structure
Dmso Reductase
Angstrom Resolution
Molybdenum Cofactor
Enzyme
Molybdopterin
Family
Site
Spectroscopy
Biochemistry & Molecular Biology
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 04:04:32 EST