Transdermal delivery of a tetrapeptide: Evaluation of passive diffusion

Benson, Heather A. E., Caccetta, Rima, Chen, Yan, Kearns, Philip and Toth, Istvan (2003) Transdermal delivery of a tetrapeptide: Evaluation of passive diffusion. Letters in Peptide Science, 10 5-6: 615-620. doi:10.1007/BF02442595

Author Benson, Heather A. E.
Caccetta, Rima
Chen, Yan
Kearns, Philip
Toth, Istvan
Title Transdermal delivery of a tetrapeptide: Evaluation of passive diffusion
Journal name Letters in Peptide Science   Check publisher's open access policy
ISSN 0929-5666
Publication date 2003-09
Year available 2004
Sub-type Article (original research)
DOI 10.1007/BF02442595
Volume 10
Issue 5-6
Start page 615
End page 620
Total pages 6
Place of publication Leiden, Netherlands
Publisher Springer
Collection year 2004
Language eng
Subject C1
250399 Organic Chemistry not elsewhere classified
780103 Chemical sciences
0399 Other Chemical Sciences
Abstract Skin penetration of the tetrapeptide Ac-Ala-Ala-Pro-Val-NH2 was assessed. This peptide sequence fits the P-P-1 subsites of elastase and inhibits human neutrophil elastase competitively. Consequently this peptide may be therapeutically useful in a variety of inflammatory disorders, including psoriasis. in which elevated levels of human neutrophil elastase have been reported. Peptide penetration was assessed across whole human skin, whole skin with the stratum corneum removed by tape stripping and epidermis, which had been removed from the dermis by heat separation. The influence of 75% aqueous ethanol as a potential penetration enhancer of the tetrapeptide across epidermis was also assessed. The tetrapeptide did not penetrate whole human skin or epidermis, even under the influence of 75% aqueous ethanol. However, when the stratum corneum was removed tetrapeptide flux of 73.39 mug cm(-2) h(-1) was achieved. The study demonstrates that the stratum corneum is the main barrier to tetrapeptide skin penetration and must be overcome if therapeutically relevant amounts of tetrapeptide are to be delivered to the skin.
Keyword Biochemistry & Molecular Biology
stratum corneum
transdermal delivery
Experimental Tumor Immunoprophylaxis
Percutaneous Peptide Immunization
Murine Hair-follicles
Promoted Growth
Q-Index Code C1
Additional Notes Journal published in 2004 and backdated

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
School of Pharmacy Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 04:03:35 EST