A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis

Demana, PH, Davies, NM, Berger, B, Vosgerau, U and Rades, T (2004) A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis. Journal of Pharmacy And Pharmacology, 56 5: 573-580. doi:10.1211/0022357023259


Author Demana, PH
Davies, NM
Berger, B
Vosgerau, U
Rades, T
Title A comparison of pseudo-ternary diagrams of aqueous mixtures of Quil A, cholesterol and phospholipid prepared by lipid-film hydration and dialysis
Journal name Journal of Pharmacy And Pharmacology   Check publisher's open access policy
ISSN 0022-3573
Publication date 2004
Sub-type Article (original research)
DOI 10.1211/0022357023259
Volume 56
Issue 5
Start page 573
End page 580
Total pages 8
Editor David Jones
Duncan Craig
Place of publication UK
Publisher Pharmaceutical Press
Collection year 2004
Language eng
Subject C1
320501 Pharmaceutical Sciences and Pharmacy
730213 Preventive medicine
Abstract Pseudo-ternary diagrams for Quil A, phospholipid (phosphatidylcholine (PC) or phosphatidylethanolamine (PE)) and cholesterol were established in order to identify combinations that result in the formation of immune-stimulating complex (ISCOM) matrices and other colloidal structures produced by these three components in aqueous systems following lipid-film hydration or dialysis (methods that can be used to produce ISCOMs). In addition, the effect of equilibration time (1 month at 4degreesC) on the structures formed by the various combinations of the three components was investigated. Depending on the ratio of Quil A, cholesterol and phospholipid, different colloidal particles, including ISCOM matrices, liposomes and ring-like micelles, were found irrespective of the preparation method used. In contrast, worm-like micelles were only observed in systems prepared by lipid-film hydration. For samples prepared by dialysis, ISCOM matrices were predominantly found near the Quil A apex of the pseudo-ternary diagram (> 50% Quil A). On the other hand, for samples prepared by lipid-film hydration, ISCOM matrices were predominantly found near the phospholipid apex of the pseudo-ternary diagram (> 50% phospholipid). The regions in the pseudo-ternary diagrams in which ISCOM matrices were observed increased following an extended equilibration time, particularly for samples prepared by lipid-film hydration. Differences were also observed between pseudoternary diagrams prepared using either PE or PC as phospholipids.
Keyword Pharmacology & Pharmacy
Immune-stimulating Complexes
Iscoms
Liposomes
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 197 Abstract Views  -  Detailed Statistics
Created: Wed, 15 Aug 2007, 04:01:36 EST