Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units

O'Carroll, M. R., Syrmis, M. W., Wainwright, C. E., Greer, R. M., Mitchell, P., Coulter, C., Sloots, T. P., Nissen, M. D. and Bell, S. C. (2004) Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units. European Respiratory Journal, 24 1: 101-106. doi:10.1183/09031936.04.00122903

Author O'Carroll, M. R.
Syrmis, M. W.
Wainwright, C. E.
Greer, R. M.
Mitchell, P.
Coulter, C.
Sloots, T. P.
Nissen, M. D.
Bell, S. C.
Title Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units
Journal name European Respiratory Journal   Check publisher's open access policy
ISSN 0903-1936
Publication date 2004-07
Sub-type Article (original research)
DOI 10.1183/09031936.04.00122903
Volume 24
Issue 1
Start page 101
End page 106
Total pages 6
Editor K. F. Rabe
P. J. Sterk
Place of publication Lausanne, Switzerland
Publisher European Respiratory Society
Collection year 2004
Language eng
Subject C1
321011 Medical Genetics
730110 Respiratory system and diseases (incl. asthma)
270303 Virology
730101 Infectious diseases
Abstract Despite recent reports of clonal strains of Pseudomonas aeruginosa in cystic fibrosis (CF) units, the need for routine microbiological surveillance remains contentious. Sputum was collected prospectively from productive patients attending the regional paediatric and adult CF units in Brisbane, Australia. All P. aeruginosa isolates were typed using pulsed-field gel electrophoresis. Spirometry, anthropometrics, hospitalisations and antibiotic sensitivity data were recorded. The first 100 sputum samples (first 50 patients at each clinic) harboured 163 isolates of P. aeruginosa. A total of 39 patients shared a common strain (pulsotype 2), 20 patients shared a strain with at least one other patient and 41 patients harboured unique strains. Eight patients shared a strain identical to a previously reported Australian transmissible strain (pulsotype 1). Compared with the unique strain group, patients harbouring pulsotype 2 were younger and had poorer lung function. Treatment requirements were similar in these two groups, as were the rates of multiresistance. In conclusion, 59% of patients harboured a clonal strain, supporting the need for routine microbiological surveillance. In contrast to previously described clonal strains, the dominant pulsotype was indistinguishable from nonclonal strains with respect to both colonial morphology and multiresistance. The clinical significance of clonal strains remains uncertain and requires longitudinal study.
Keyword Clonal Strain
Cystic Fibrosis
Pseudomonas Aerugoinosa
Field Gel Electrophoresis
Respiratory System
Pseudomonas Aeruginosa
Pulsed-field Gel Electrophoresis
Transmissible Strain
Q-Index Code C1

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