Homologues of the maize rust resistance gene Rp1-D are genetically associated with a major rust resistance QTL in sorghum

McIntyre, C. L., Hermann, S. M., Casu, R. E., Knight, D., Drenth, J., Tao, Y., Brumbley, S. M., Godwin, I. D., Williams, S., Smith, G. R. and Manners, J. M. (2004) Homologues of the maize rust resistance gene Rp1-D are genetically associated with a major rust resistance QTL in sorghum. Theoretical and Applied Genetics, 109 4: 875-883. doi:10.1007/s00122-004-1702-0


Author McIntyre, C. L.
Hermann, S. M.
Casu, R. E.
Knight, D.
Drenth, J.
Tao, Y.
Brumbley, S. M.
Godwin, I. D.
Williams, S.
Smith, G. R.
Manners, J. M.
Title Homologues of the maize rust resistance gene Rp1-D are genetically associated with a major rust resistance QTL in sorghum
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
1432-2242
Publication date 2004
Sub-type Article (original research)
DOI 10.1007/s00122-004-1702-0
Volume 109
Issue 4
Start page 875
End page 883
Total pages 9
Editor H. C. Becker
Place of publication Berlin
Publisher Springer-Verlag
Collection year 2004
Language eng
Subject C1
300201 Plant Biochemistry and Physiology
620104 Other cereals
Abstract As part of a comparative mapping study between sugarcane and sorghum, a sugarcane cDNA clone with homology to the maize Rp1-D rust resistance gene was mapped in sorghum. The cDNA probe hybridised to multiple loci, including one on sorghum linkage group (LG) E in a region where a major rust resistance QTL had been previously mapped. Partial sorghum Rp1-D homologues were isolated from genomic DNA of rust-resistant and -susceptible progeny selected from a sorghum mapping population. Sequencing of the Rp1-D homologues revealed five discrete sequence classes: three from resistant progeny and two from susceptible progeny. PCR primers specific to each sequence class were used to amplify products from the progeny and confirmed that the five sequence classes mapped to the same locus on LG E. Cluster analysis of these sorghum sequences and available sugarcane, maize and sorghum Rp1-D homologue sequences showed that the maize Rp1-D sequence and the partial sugarcane Rp1-D homologue were clustered with one of the sorghum resistant progeny sequence classes, while previously published sorghum Rp1-D homologue sequences clustered with the susceptible progeny sequence classes. Full-length sequence information was obtained for one member of a resistant progeny sequence class (Rp1-SO) and compared with the maize Rp1-D sequence and a previously identified sorghum Rp1 homologue (Rph1-2). There was considerable similarity between the two sorghum sequences and less similarity between the sorghum and maize sequences. These results suggest a conservation of function and gene sequence homology at the Rp1 loci of maize and sorghum and provide a basis for convenient PCR-based screening tools for putative rust resistance alleles in sorghum.
Keyword Agronomy
Plant Sciences
Genetics & Heredity
Horticulture
Bicolor L. Moench
Disease Resistance
Rflp Map
Molecular Characterization
Genomic Regions
Inbred Lines
Linkage-map
Markers
Locus
Identification
Q-Index Code C1

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 03:54:42 EST