Immunohistological analysis of Tannerella forsythia-induced lesions in a murine model

Gosling, P. T., Gemmell, E., Carter, C. L., Bird, P. S. and Seymour, G. J. (2005) Immunohistological analysis of Tannerella forsythia-induced lesions in a murine model. Oral Microbiology And Immunology, 20 1: 25-30. doi:10.1111/j.1399-302X.2004.00188.x


Author Gosling, P. T.
Gemmell, E.
Carter, C. L.
Bird, P. S.
Seymour, G. J.
Title Immunohistological analysis of Tannerella forsythia-induced lesions in a murine model
Journal name Oral Microbiology And Immunology   Check publisher's open access policy
ISSN 0902-0055
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1111/j.1399-302X.2004.00188.x
Volume 20
Issue 1
Start page 25
End page 30
Total pages 6
Place of publication Denmark
Publisher Blackwell Munskgaard
Collection year 2005
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
1105 Dentistry
Abstract Tannerella forsythia has been implicated as a defined periodontal pathogen. In the present study a mouse model was used to determine the phenotype of leukocytes in the lesions induced by subcutaneous injections of either live (group A) or nonviable (group B) T. forsythia. Control mice (group C) received the vehicle only. Lesions were excised at days 1, 2, 4, and 7. An avidin-biotin immunoperoxidase method was used to stain infiltrating CD4(+) and CD8(+) T cells, CD14(+) macrophages, CD19(+) B cells, and neutrophils. Hematoxylin and eosin sections demonstrated lesions with central necrotic cores surrounded by neutrophils, macrophages and lymphocytes in both group A and group B mice. Lesions from control mice exhibited no or only occasional solitary leukocytes. In both groups A and B, neutrophils were the dominant leukocyte in the lesion 1 day after injection, the numbers decreasing over the 7-day experimental period. There was a relatively low mean percent of CD4(+) and CD8(+) T cells in the lesions and, whereas the percent of CD8(+) T cells remained constant, there was a significant increase in the percent of CD4(+) T cells at day 7. This increase was more evident in group A mice. The mean percent of CD14(+) macrophages and CD19(+) B cells remained low over the experimental period, although there was a significantly higher mean percent of CD19(+) B cells at day 1. In conclusion, the results showed that immunization of mice with live T. forsythia induced a stronger immune response than nonviable organisms. The inflammatory response presented as a nonspecific immune response with evidence of an adaptive (T-cell) response by day 7. Unlike Porphyromonas gingivalis, there was no inhibition of neutrophil migration.
Keyword Dentistry, Oral Surgery & Medicine
Immunology
Microbiology
Immunohistology
Leukocytes
Mouse Model
Tannerella Forsythia
Porphyromonas-gingivalis
Bacteroides-forsythus
Periodontal-disease
Mixed Infection
S-layer
Virulence
Colonization
Immunization
Microbiota
Responses
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2006 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Wed, 15 Aug 2007, 13:51:34 EST