Characterisation of human kallikrein 6 protease M expression in ovarian cancer

Ni, X., Zhang, W., Huang, K. C., Wang, Y., Ng, S. K., Mok, S. C., Berkowitz, R. S. and Ng, S. W. (2004) Characterisation of human kallikrein 6 protease M expression in ovarian cancer. British Journal of Cancer, 91 4: 725-731. doi:10.1038/sj.bjc.6602041


Author Ni, X.
Zhang, W.
Huang, K. C.
Wang, Y.
Ng, S. K.
Mok, S. C.
Berkowitz, R. S.
Ng, S. W.
Title Characterisation of human kallikrein 6 protease M expression in ovarian cancer
Journal name British Journal of Cancer   Check publisher's open access policy
ISSN 0007-0920
1532-1827
Publication date 2004
Sub-type Article (original research)
DOI 10.1038/sj.bjc.6602041
Open Access Status DOI
Volume 91
Issue 4
Start page 725
End page 731
Total pages 7
Editor A.L. Harris
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2004
Language eng
Abstract Kallikrein 6 (hK6, also known as protease M/zyme/neurosin) is a member of the human kallikrein gene family. We have previously cloned the cDNA for this gene by differential display and shown the overexpression of the mRNA in breast and ovarian primary tumour tissues and cell lines. To thoroughly characterise the expression of this kallikrein in ovarian cancer, we have developed a novel monoclonal antibody specific to hK6 and employed it in immunohistochemistry with a wide range of ovarian tumour samples. The expression was found elevated in 67 of 80 cases of ovarian tumour samples and there was a significant difference in the expression levels between normal and benign ovarian tissues and the borderline and invasive tumours (P<0.001). There was no difference of expression level between different subtypes of tumours. More significantly, high level of kallikrein 6 expression was found in many early-stage and low-grade tumours, and elevated hK6 proteins were found in benign epithelia coexisting with borderline and invasive tissues, suggesting that overexpression of hK6 is an early phenomenon in the development of ovarian cancer. Quantitative real-time reverse transcription-polymerase chain reactions also showed elevated kallikrein 6 mRNA expression in ovarian tumours. Genomic Southern analysis of 19 ovarian tumour samples suggested that gene amplification is one mechanism for the overexpression of hK6 in ovarian cancer.
Keyword Oncology
Serine protease
Monoclonal antibody
Ovarian cancer
Biomarker
Gene amplification
Prostate-specific antigen
Tumor-markers
Carcinoma
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Physical Sciences Publications
 
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Created: Wed, 15 Aug 2007, 03:26:54 EST