Cambial meristem dormancy in trees involves extensive remodelling of the transcriptome

Schrader, Jarmo, Moyle, Richard, Bhalerao, Rupali, Hertzberg, Magnus, Lundeberg, Joakim, Nilsson, Peter and Bhalerao, Rishikesh P. (2004) Cambial meristem dormancy in trees involves extensive remodelling of the transcriptome. The Plant Journal, 40 2: 173-187. doi:10.1111/j.1365-313X.2004.02199.x

Author Schrader, Jarmo
Moyle, Richard
Bhalerao, Rupali
Hertzberg, Magnus
Lundeberg, Joakim
Nilsson, Peter
Bhalerao, Rishikesh P.
Title Cambial meristem dormancy in trees involves extensive remodelling of the transcriptome
Journal name The Plant Journal   Check publisher's open access policy
ISSN 0960-7412
Publication date 2004-10
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2004.02199.x
Volume 40
Issue 2
Start page 173
End page 187
Total pages 15
Editor D. Bowles
Place of publication Oxford
Publisher BIOS Publishers : Blackwell Scientific in association with the Society for Experimental Biology
Collection year 2004
Language eng
Subject C1
270201 Gene Expression
620300 Forestry
Abstract The establishment of the dormant state in meristems involves considerable physiological and metabolic alterations necessary for surviving unfavourable growth conditions. However, a global molecular analysis of dormancy in meristems has been hampered by the difficulty in isolating meristem cells. We used cryosectioning to isolate purified cambial meristem cells from the woody plant Populus tremula during active growth and dormancy. These samples were used to generate meristem-specific cDNA libraries and for cDNA microarray experiments to define the global transcriptional changes underlying cambial dormancy. The results indicate a significant reduction in the complexity of the cambial transcriptome in the dormant state. Although cell division is terminated in the dormant cambium, the cell cycle machinery appears to be maintained in a skeletal state as suggested by the continued presence of transcripts for several cell cycle regulators. The downregulation of PttPIN1 and PttPIN2 transcripts explains the reduced basipetal polar auxin transport during dormancy. The induction of a member of the SINA family of ubiquitin ligases implicated in auxin signalling indicates a potential mechanism for modulation of auxin sensitivity during cambial dormancy. The metabolic alterations during dormancy are mirrored in the induction of genes involved in starch breakdown and the glyoxysomal cycle. Interestingly, the induction of RGA1 like gene suggests modification of gibberellin signalling in cambial dormancy. The induction of genes such as poplar orthologues of FIE and HAP2 indicates a potential role for these global regulators of transcription in orchestrating extensive changes in gene expression during dormancy.
Keyword dormancy
populus tremula
wood formation
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 03:22:23 EST