Cross-reactive recognition of human and primate cytomegalovirus sequences by human CD4 cytotoxic T lymphocytes specific for glycoprotein B and H

Elkington, Rebecca, Shoukry, Naglaa H., Walker, Susan, Crough, Tania, Fazou, Chrysa, Kaur, Amitinder, Walker, Christopher M. and Khanna, Rajiv (2004) Cross-reactive recognition of human and primate cytomegalovirus sequences by human CD4 cytotoxic T lymphocytes specific for glycoprotein B and H. European Journal of Immunology, 34 11: 3216-3226. doi:10.1002/eji.200425203


Author Elkington, Rebecca
Shoukry, Naglaa H.
Walker, Susan
Crough, Tania
Fazou, Chrysa
Kaur, Amitinder
Walker, Christopher M.
Khanna, Rajiv
Title Cross-reactive recognition of human and primate cytomegalovirus sequences by human CD4 cytotoxic T lymphocytes specific for glycoprotein B and H
Journal name European Journal of Immunology   Check publisher's open access policy
ISSN 0014-2980
Publication date 2004
Sub-type Article (original research)
DOI 10.1002/eji.200425203
Volume 34
Issue 11
Start page 3216
End page 3226
Total pages 11
Editor L. Leclercq Reth
Place of publication Weinheim, Germany
Publisher Wiley-VCH Verlag GmbH & Co. KGaA
Collection year 2004
Language eng
Subject C1
320202 Cellular Immunology
730102 Immune system and allergy
Abstract Although the importance of CD4(+) T cell responses to human cytonnegalovirus (HCMV) has recently been recognized in transplant and immunosuppressed patients, the precise specificity and nature of this response has remained largely unresolved. In the present study we have isolated CD4(+) CTL which recognize epitopes from HCMV glycoproteins gB and gH in association with two different HLA-DR antigens, DRA1*0101/DRB1*0701 (DR7) and DRA1*0101/DRB1*1101 (DR11). Comparison of amino acid sequences of HICMV isolates revealed that the gB and gH epitope sequences recognized by human CD4(+) T cells were not only conserved in clinical isolates from HCMV but also in CMV isolates from higher primates (chimpanzee, rhesus and baboon). Interestingly, these epitope sequences from chimpanzee, rhesus and baboon CMV are efficiently recognized by human CD4(+) CTL. More importantly, we show that gB-specific T cells from humans can also efficiently lyse pepticle-sensitized Patr-DR7(+) cells from chimpanzees. These findings suggest that conserved gB and gH epitopes should be considered while designing a prophylactic vaccine against HCMV. In addition, they also provide a functional basis for the conservation of MHC class 11 lineages between humans and Old World primates and open the possibility for the use of such primate models in vaccine development against HCMV.
Keyword Immunology
Cd4(+) T Cells
Human Cmv
Glycoprotein
Ctl
Primate Cmv
Recombinant Vaccinia Infection
Cell Epitopes
Protein Pp65
Peptide Binding
Ctl Response
Virus
Complex
Chimpanzee
Identification
Vivo
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 03:06:25 EST