The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein

Krauer, Kenia G., Buck, Marion, Belzer, Deanna K., Flanagan, James, Chojnowski, Grace M. and Sculley, Tom B. (2004) The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein. Virology, 318 1: 280-294. doi:10.1016/j.virol.2003.09.032


Author Krauer, Kenia G.
Buck, Marion
Belzer, Deanna K.
Flanagan, James
Chojnowski, Grace M.
Sculley, Tom B.
Title The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
Publication date 2004
Sub-type Article (original research)
DOI 10.1016/j.virol.2003.09.032
Volume 318
Issue 1
Start page 280
End page 294
Total pages 15
Editor R. Lamb
Place of publication San Diego, U.S.A.
Publisher Academic Press
Collection year 2004
Language eng
Subject C1
320305 Medical Biochemistry - Proteins and Peptides
730108 Cancer and related disorders
Abstract The Epstein-Barr virus nuclear antigen (EBNA)-6 protein is essential for Epstein-Barr virus (EBV)-induced immortalization of primary human B-lymphocytes in vitro. In this study, fusion proteins of EBNA-6 with green fluorescent protein (GFP) have been used to characterize its nuclear localization and organization within the nucleus. EBNA-6 associates with nuclear structures and in immunofluorescence demonstrate a punctate staining pattern. Herein, we show that the association of EBNA-6 with these nuclear structures was maintained throughout the cell cycle and with the use of GFP-E6 deletion mutants, that the region amino acids 733-808 of EBNA-6 contains a domain that can influence the association of EBNA-6 with these nuclear structures. Co-immunofluorescence and confocal analyses demonstrated that EBNA-6 and EBNA-3 co-localize in the nucleus of cells. Expression of EBNA-6, but not EBNA-3, caused a redistribution of nuclear survival of motor neurons protein (SMN) to the EBNA-6 containing nuclear structures resulting in co-localization of SMN with EBNA-6. (C) 2003 Elsevier Inc. All rights reserved.
Keyword Virology
Ebv
Ebna-6
Ebna-3
Nuclear Localization
Nuclear Structures
Smn
Gfp
Gems
Dead Box Protein
Prothymosin-alpha
Binding Protein
Coiled Bodies
Gene-product
J-kappa
3c
Transcription
Localization
Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 03:05:56 EST