Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco

Ramage, CM and Williams, RR (2004) Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco. Plant Cell Reports, 22 12: 919-924. doi:10.1007/s00299-004-0774-2


Author Ramage, CM
Williams, RR
Title Cytokinin-induced abnormal shoot organogenesis is associated with elevated Knotted1-type homeobox gene expression in tobacco
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 2004
Sub-type Article (original research)
DOI 10.1007/s00299-004-0774-2
Volume 22
Issue 12
Start page 919
End page 924
Total pages 6
Editor G. Hahne
O. L. Gamborg
Place of publication Germany
Publisher Springer-Verlag
Collection year 2004
Language eng
Subject C1
300302 Plant Growth and Development
620403 Tobacco leaf
Abstract The molecular mechanisms that regulate the transcription of key developmental genes involved in shoot organogenesis have yet to be fully elucidated. However, it is clear that plant growth regulators, such as cytokinin, play a critical role in the differentiation of adventitious shoots. In Nicotiana tabacum zz100 leaf discs, high frequency shoot formation could be induced with 5 muM of the cytokinin N-6-benzyladenine (BA). Increasing the exogenous BA concentration to greater than 20 muM resulted in stunted explants with abnormal shoot morphology and altered mineral composition. Explants with abnormal shoots did not appear to be hyperhydric. Abnormalities were, however, associated with an increase in the expression of a knotted1-type homeobox gene (TobH1) isolated from normal shoot-forming cultures. The results suggest that the development of cytokinin-induced abnormal shoot morphology possibly involves changes in TobH1 gene expression.
Keyword Plant Sciences
Knotted-1 Homeobox
Tissue Culture
N-6-benzyladenine
Abnormal Shoot Morphology
Transgenic Tobacco
Altered Morphology
Ectopic Expression
Hormone-levels
Plant-cells
Protein
Growth
Arabidopsis
Tissues
Leaves
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 03:03:10 EST