Development and transplantation of a mineralized matrix formed by osteoblasts in vitro for bone regeneration

Xiao, Y, Haase, H, Young, WG and Bartold, PM (2004) Development and transplantation of a mineralized matrix formed by osteoblasts in vitro for bone regeneration. Cell Transplantation, 13 1: 15-25.

Author Xiao, Y
Haase, H
Young, WG
Bartold, PM
Title Development and transplantation of a mineralized matrix formed by osteoblasts in vitro for bone regeneration
Journal name Cell Transplantation   Check publisher's open access policy
ISSN 0963-6897
Publication date 2004
Sub-type Article (original research)
Volume 13
Issue 1
Start page 15
End page 25
Total pages 11
Editor Dr Paul Sanberg
Place of publication USA
Publisher Cognizant Communication Corp
Collection year 2004
Language eng
Subject C1
320899 Dentistry not elsewhere classified
Abstract The use of extracellular matrix materials as scaffolds for the repair and regeneration of tissues is receiving increased attention. The current study was undertaken to test whether extracellular matrix formed by osteoblasts in vitro could be used as a scaffold for osteoblast transplantation and induce new bone formation in critical size osseous defects in vivo. Human osteoblasts derived from alveolar bone were cultured in six-well plates until confluent and then in mineralization media for a further period of 3 weeks to form an osteoblast-mineralized matrix complex. Histologically, at this time point a tissue structure with a connective tissue-like morphology was formed. Type I collagen was the major extracellular component present and appeared to determine the matrix macrostructure. Other bone-related proteins such as alkaline phosphatase (ALP), bone morphogenetic protein (BMP)-2 and -4, bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) also accumulated in the matrix. The osteoblasts embedded in this matrix expressed mRNAs for these bone-related proteins very strongly. Nodules of calcification were detected in the matrix and there was a correlation between calcification and the distribution of BSP and OPN. When this matrix was transplanted into a critical size bone defect in skulls of inummodeficient mice (SCID), new bone formation occurred. Furthermore, the cells inside the matrix survived and proliferated in the recipient sites, and were traceable by the human-specific Alu gene sequence using in situ hybridization. It was found that bone-forming cells differentiated from both transplanted human osteoblasts and activated endogenous mesenchymal cells. This study indicates that a mineralized matrix, formed by human osteoblasts in vitro, can be used as a scaffold for osteoblast transplantation, which subsequently can induce new bone formation.
Keyword Cell Biology
Extracellular Matrix
Mineralized Matrix
Bone Formation
Induced Osteogenesis
Polymer Scaffolds
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 40 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 02:58:25 EST