Simple In Vitro Assay for Determining the Sensitivity of Plasmodium vivax Isolates from Fresh Human Blood to Antimalarials in Areas where P. vivax Is Endemic

Russell, BM, Udomsangpetch, R, Rieckmann, KH, Kotecka, BA, Coleman, RE and Sattabongkot, J (2003) Simple In Vitro Assay for Determining the Sensitivity of Plasmodium vivax Isolates from Fresh Human Blood to Antimalarials in Areas where P. vivax Is Endemic. Antimicrobial Agents And Chemotherapy, 47 1: 170-173. doi:10.1128/AAC.47.1.170-173.2003

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Author Russell, BM
Udomsangpetch, R
Rieckmann, KH
Kotecka, BA
Coleman, RE
Sattabongkot, J
Title Simple In Vitro Assay for Determining the Sensitivity of Plasmodium vivax Isolates from Fresh Human Blood to Antimalarials in Areas where P. vivax Is Endemic
Journal name Antimicrobial Agents And Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
Publication date 2003
Sub-type Article (original research)
DOI 10.1128/AAC.47.1.170-173.2003
Open Access Status File (Publisher version)
Volume 47
Issue 1
Start page 170
End page 173
Total pages 4
Editor G.M. Eliopoulos
Place of publication United States
Publisher American Society for Microbiology
Collection year 2003
Language eng
Subject C1
321202 Epidemiology
730212 Disease distribution and transmission
Abstract The aim of this study was to develop a simple, field-practical, and effective in vitro method for determining the sensitivity of fresh erythrocytic Plasmodium vivax isolates to a range of antimalarials. The method used is a modification of the standard World Health Organization (WHO) microtest for determination of P.falciparum drug sensitivity. The WHO method was modified by removing leukocytes and using a growth medium supplemented with AB(+) serum. We successfully carried out 34 in vitro drug assays on 39 P. vivax isolates collected from the Mae Sod malaria clinic, Tak Province, Thailand. The mean percentage of parasites maturing to schizonts (six or more merozoites) in control wells was 66.5% +/- 5.9% (standard deviation). This level of growth in the control wells enabled rapid microscopic determination (5 min per isolate per drug) of the MICs of chloroquine, dihydroartemisinin, WR238605 (tafenoquine), and sulfadoxine. P. vivax was relatively sensitive to chloroquine (MIC = 160 ng/ml, 50% inhibitory concentration [IC50] = 49.8 ng/ml) and dihydroartemisinin (MIC = 0.5 ng/ml, IC50 = 0.47 ng/ml). The poor response of P. vivax to both tafenoquine (MIC = 14,000 ng/ml, IC50 = 9,739 ng/ml) and sulfadoxine (MIC = 500,000 ng/ml, IC50 = 249,000 ng/ml) was due to the slow action of these drugs and the innate resistance of P. vivax to sulfadoxine. The in vitro assay developed in our study should be useful both for assessing the antimalarial sensitivity of P. vivax populations and for screening new antimalarials in the absence of long-term P. vivax cultures.
Keyword Microbiology
Pharmacology & Pharmacy
Chloroquine
Resistance
Falciparum
Malaria
Efflux
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Public Health Publications
 
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Created: Wed, 15 Aug 2007, 02:55:11 EST