Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

Kotecka, BM, Rieckmann, KH, Davis, TME, Batty, KT and Ilett, KF (2003) Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma. Acta Tropica, 87 3: 371-375. doi:10.1016/S0001-706X(03)00118-9


Author Kotecka, BM
Rieckmann, KH
Davis, TME
Batty, KT
Ilett, KF
Title Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma
Journal name Acta Tropica   Check publisher's open access policy
ISSN 0001-706X
Publication date 2003
Sub-type Article (original research)
DOI 10.1016/S0001-706X(03)00118-9
Volume 87
Issue 3
Start page 371
End page 375
Total pages 5
Editor A. Bjorkman
K. Berzins
Place of publication Netherlands
Publisher Elsevier
Collection year 2003
Language eng
Subject C1
321202 Epidemiology
730212 Disease distribution and transmission
Abstract The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.
Keyword Parasitology
Tropical Medicine
Malaria
Artesunate
Dihydroartemisinin
Bioassay
Hplc
Uncomplicated Falciparum-malaria
Oral Artesunate
Pharmacokinetics
Artemether
Culture
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
Centre for Military and Veterans' Health Publications
 
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Created: Wed, 15 Aug 2007, 02:55:07 EST