Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineage

Wells, C. A., Ravasi, T., Sultana, R., Yagi, K., Carninci, P., Bono, H., Faulkner, G. J., Okazaki, Y., Quackenbush, J., Hume, D. A., RIKEN GER Group,, GSL Members, and Lyons, P. A. (2003) Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineage. Genome Research, 13 6B: 1360-1365. doi:10.1101/gr.1056103

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Author Wells, C. A.
Ravasi, T.
Sultana, R.
Yagi, K.
Carninci, P.
Bono, H.
Faulkner, G. J.
Okazaki, Y.
Quackenbush, J.
Hume, D. A.
GSL Members,
Lyons, P. A.
Title Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineage
Journal name Genome Research   Check publisher's open access policy
ISSN 1088-9051
Publication date 2003
Sub-type Article (original research)
DOI 10.1101/gr.1056103
Open Access Status File (Publisher version)
Volume 13
Issue 6B
Start page 1360
End page 1365
Total pages 6
Place of publication Cold Spring Harbor, NY 11724 United States
Publisher Cold Spring Harbor Press
Collection year 2003
Language eng
Abstract The current RIKEN transcript set represents a significant proportion of the mouse transcriptome but transcripts expressed in the innate and acquired immune systems are poorly represented. In the present study we have assessed the complexity of the transcriptome expressed in mouse macrophages before and after treatment with lipopolysaccharide, a global regulator of macrophage gene expression, using existing RIKEN 19K arrays. By comparison to array profiles of other cells and tissues, we identify a large set of macrophage-enriched genes, many of which have obvious functions in endocytosis and phagocytosis. In addition, a significant number of LPS-inducible genes were identified. The data suggest that macrophages are a complex source of mRNA for transcriptome studies. To assess complexity and identify additional macrophage expressed genes, cDNA libraries were created from purified populations of macrophage and dendritic cells, a functionally related cell type. Sequence analysis revealed a high incidence of novel mRNAs within these cDNA libraries. These studies provide insights into the depths of transcriptional complexity still untapped amongst products of inducible genes, and identify macrophage and dendritic cell populations as a starting point for sampling the inducible mammalian transcriptome.
Keyword Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Genetics & Heredity
Functional Annotation
Human Genome
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 33 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 36 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 02:52:05 EST