Regulation of the murine TRACP gene promoter

Cassady, A. I., Luchin, A., Ostrowski, M. C. and Hume, David A. (2003) Regulation of the murine TRACP gene promoter. Journal of Bone And Mineral Research, 18 10: 1901-1904. doi:10.1359/jbmr.2003.18.10.1901

Author Cassady, A. I.
Luchin, A.
Ostrowski, M. C.
Hume, David A.
Title Regulation of the murine TRACP gene promoter
Journal name Journal of Bone And Mineral Research   Check publisher's open access policy
ISSN 0884-0431
Publication date 2003-10
Sub-type Article (original research)
DOI 10.1359/jbmr.2003.18.10.1901
Volume 18
Issue 10
Start page 1901
End page 1904
Total pages 4
Place of publication Washington
Publisher American Society for Bone and Mineral Research
Collection year 2003
Language eng
Subject C1
270202 Genome Structure
730114 Skeletal system and disorders (incl. arthritis)
Abstract The activity of the TRACP promoter has been investigated as a model of gene regulation in osteoclasts. The murine TRACP gene promoter contains potential binding sites for a number of transcription factors in particular, candidate sites for the Ets factor PU.1 and for the microphthalmia transcription factor (MiTF). These are of relevance to osteoclast biology because the PU.1 knockout mouse has an osteopetrotic phenotype, and MiTF, when mutated in the mi/mi mouse, also results in osteopetrosis. The binding sites for both of these factors have been identified, and they have been determined to be functional in regulating TRACP expression. A novel assay system using the highly osteoclastogenic RAW/C4 subclone of the murine macrophage cell line RAW264.7 was used to perform gene expression experiments on macrophage and osteoclast cell backgrounds. We have shown that TRACP expression is a target for regulation by the macrophage/osteoclast transcription factor PU.1 and the osteoclast commitment factor MiTF and that these factors act synergistically in regulating this promoter. This directly links two controlling factors of osteoclast differentiation to the expression of an effector of cell function.
Keyword Endocrinology & Metabolism
Gene Expression
Microphthalmia Transcription Factor
Resistant Acid-phosphatase
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 02:51:36 EST