Investigation of candidate division TM7, a recently recognized major lineage of the domain bacteria with no known pure-culture representatives

Hugenholtz, Philip, Tyson, Gene W., Webb, Richard I., Wagner, Ankia M. and Blackall, Linda L. (2001) Investigation of candidate division TM7, a recently recognized major lineage of the domain bacteria with no known pure-culture representatives. Applied And Environmental Microbiology, 67 1: 411-419. doi:10.1128/AEM.67.1.411-419.2001

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Author Hugenholtz, Philip
Tyson, Gene W.
Webb, Richard I.
Wagner, Ankia M.
Blackall, Linda L.
Title Investigation of candidate division TM7, a recently recognized major lineage of the domain bacteria with no known pure-culture representatives
Journal name Applied And Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2001-01
Sub-type Article (original research)
DOI 10.1128/AEM.67.1.411-419.2001
Open Access Status File (Publisher version)
Volume 67
Issue 1
Start page 411
End page 419
Total pages 9
Place of publication Washington, D.C. U.S.A.
Publisher American Society of Microbiology
Collection year 2001
Language eng
Subject C1
270307 Microbial Ecology
770502 Land and water management
Abstract A molecular approach was used to investigate a recently described candidate division of the domain Bacteria, TM7, currently known only from environmental 16S ribosomal DNA sequence data, A number of TM7-specific primers and probes were designed and evaluated. Fluorescence in situ hybridization (FISH) of a laboratory scale bioreactor using two independent TM7-specific probes revealed a conspicuous sheathed-filament morphotype, fortuitously enriched in the reactor. Morphologically, the filament matched the description of the Eikelboom morphotype 0041-0675 widely associated with bulking problems in activated-sludge wastewater treatment systems. Transmission electron microscopy of the bioreactor sludge demonstrated that the sheathed-filament morphotype had a typical gram-positive cell envelope ultrastructure. Therefore, TM7 is only the third bacterial lineage recognized to have gram-positive representatives. TM7-specific FISH analysis of two full-scale wastewater treatment plant sludges, including the one used to seed the laboratory scale reactor, indicated the presence of a number of morphotypes, including sheathed filaments. TM7-specific PCR clone libraries prepared from the two full-scale sludges yielded 23 novel TM7 sequences. Three subdivisions could be defined based on these data and publicly available sequences. Environmental sequence data and TM7-specific FISH analysis indicate that members of the TM7 division are present in a variety of terrestrial, aquatic, and clinical habitats. A highly atypical base substitution (Escherichia coli position 912; C to U) for bacterial 16S rRNAs was present in almost all TM7 sequences, suggesting that TM7 bacteria, like Archaea, may be streptomycin resistant at the ribosome level.
Keyword Biotechnology & Applied Microbiology
Microbiology
Microbial Diversity
Activated-sludge
Identification
Search
Probes
View
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Tue, 14 Aug 2007, 19:40:31 EST