Duodenal expression of iron transport molecules in untreated haemochromatosis subjects

Stuart, KA, Anderson, GJ, Frazer, DM, Powell, LW, McCullen, M, Fletcher, LM and Crawford, DHG (2003) Duodenal expression of iron transport molecules in untreated haemochromatosis subjects. Gut, 52 7: 953-959. doi:10.1136/gut.52.7.953

Author Stuart, KA
Anderson, GJ
Frazer, DM
Powell, LW
McCullen, M
Fletcher, LM
Crawford, DHG
Title Duodenal expression of iron transport molecules in untreated haemochromatosis subjects
Journal name Gut   Check publisher's open access policy
ISSN 0017-5749
Publication date 2003
Sub-type Article (original research)
DOI 10.1136/gut.52.7.953
Volume 52
Issue 7
Start page 953
End page 959
Total pages 7
Editor Robin C Spiller
Place of publication United Kingdom
Publisher BMJ Publishing Group
Collection year 2003
Language eng
Subject C1
321006 Gastroenterology and Hepatology
730113 Digestive system and disorders
Abstract Background and aims: In HFE associated hereditary haemochromatosis, the duodenal enterocyte behaves as if iron deficient and previous reports have shown increased duodenal expression of divalent metal transporter 1 (DMT1) and iron regulated gene 1 (Ireg1) in affected subjects. In those studies, many patients had undergone venesection, which is a potent stimulus of iron absorption. Our study investigated duodenal expression of DMT1 ( IRE and non-IRE), Ireg1, hephaestin, and duodenal cytochrome-b (Dyctb) in untreated C282Y homozygous haemochromatosis patients, iron deficient patients, and iron replete subjects. Methods: Total RNA was extracted from duodenal biopsies and expression of the iron transport genes was assessed by ribonuclease protection assay. Results: Expression of DMT1 ( IRE) and Ireg1 was increased 3 - 5-fold in iron deficient subjects compared with iron replete subjects. Duodenal expression of DMT1 ( IRE) and Ireg1 was similar in haemochromatosis patients and iron replete subjects but in haemochromatosis patients with elevated serum ferritin concentrations, both DMT1 ( IRE) and Ireg1 expression were inappropriately increased relative to serum ferritin concentration. Hephaestin and Dcytb levels were not upregulated in haemochromatosis. DMT1 ( IRE) and Ireg1 levels showed significant inverse correlations with serum ferritin concentration in each group of patients. Conclusions: These findings are consistent with DMT1 ( IRE) and Ireg1 playing primary roles in the adaptive response to iron deficiency. Untreated haemochromatosis patients showed inappropriate increases in DMT1 ( IRE) and Ireg1 expression for a given level of serum ferritin concentration, although the actual level of expression of these iron transport genes was not significantly different from that of normal subjects.
Keyword Gastroenterology & Hepatology
Australian Hemochromatosis Patients
Hereditary Hemochromatosis
Idiopathic Hemochromatosis
Food Iron
Genetic Hemochromatosis
Clinical Expression
Mucosal Abnormality
Animal Proteins
Serum Ferritin
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 39 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 43 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 02:46:29 EST