Granulocyte-colony stimulating factor increases CD123hi blood dendritic cells with altered CD62L and CCR7 expression

Vuckovic, S., Kim, M., Turtle, C. J., Khalil, D., Crosbie, G.V., Williams, K., Williams, N., Brown, L., Kelly, C., Stravos, P., Rodwell, R., Hill, G., Wright, S. J., Taylor, K., Gill, D., Marlton, P., Bradstock, K. and Hart, D. N. J. (2003) Granulocyte-colony stimulating factor increases CD123hi blood dendritic cells with altered CD62L and CCR7 expression. Blood, 101 6: 2314-2317. doi:10.1182/blood-2002-03-0973


Author Vuckovic, S.
Kim, M.
Turtle, C. J.
Khalil, D.
Crosbie, G.V.
Williams, K.
Williams, N.
Brown, L.
Kelly, C.
Stravos, P.
Rodwell, R.
Hill, G.
Wright, S. J.
Taylor, K.
Gill, D.
Marlton, P.
Bradstock, K.
Hart, D. N. J.
Title Granulocyte-colony stimulating factor increases CD123hi blood dendritic cells with altered CD62L and CCR7 expression
Formatted title
Granulocyte-colony stimulating factor increases CD123hi blood dendritic cells with altered CD62L and CCR7 expression
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
Publication date 2003-03-15
Sub-type Article (original research)
DOI 10.1182/blood-2002-03-0973
Volume 101
Issue 6
Start page 2314
End page 2317
Total pages 4
Editor S. J. Shattil
Place of publication United States
Publisher American Society of Hematology
Collection year 2003
Language eng
Subject C1
320206 Tumor Immunology
730102 Immune system and allergy
Abstract Changes in blood dendritic cell (BDC) counts (CD123(hi)BDC and CD11c(+)BDC) and expression of CD62L, CCR7, and CD49d were analyzed in healthy donors, multiple myeloma (MM), and non-Hodgkin lymphoma (NHL) patients, who received granulocyte-colony stimulating factor (G-CSF) containing peripheral blood stem cell (PBSC) mobilization protocols. Low-dose G-CSF in healthy donors (8-10 mug/ kg/d subcutaneously) and high-dose G-CSF in patients (30 mug/kg/d) increased CD123(hi)BDC (2- to 22-fold, mean 3.7 x 10(6)/ L-17.7 x 10(6)/L and 1.9 x 10(6)/L-12.0 x 10(6)/ L) in healthy donors and MM but decreased CD11c(+)BDC (2- to 10-fold, mean 5.7 x 10(6)/L-1.6 x 10(6)/L) in NHL patients, on the day of apheresis, compared with steady state. After apheresis, CD123(hi)BDC counts remained high, whereas low CD11c(+)BDC counts tended to recover in the following 2-5 days. Down-regulation of CD62L and up-regulation of CCR7 on CD123(hi)BDC were found in most healthy donors and MM patients. CD49d expression was unchanged. Thus, PBSC mobilization may change BDC counts by altering molecules necessary for BDC homing from blood into tissues.
Keyword Leukemia Hematopoietic Progenitors
Human Peripheral-blood
Marrow Stroma
In-vivo
Integrin
Mobilization
Adhesion
Interferon
Receptor
Trafficking
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 02:43:57 EST