Angiotensinogen localization and secretion in the rat pancreas

Regoli, M., Bendayan, M., Fonzi, L., Sernia, C. and Bertelli, E. (2003) Angiotensinogen localization and secretion in the rat pancreas. Journal of Endocrinology, 179 1: 81-89.


Author Regoli, M.
Bendayan, M.
Fonzi, L.
Sernia, C.
Bertelli, E.
Title Angiotensinogen localization and secretion in the rat pancreas
Journal name Journal of Endocrinology   Check publisher's open access policy
ISSN 0022-0795
Publication date 2003
Sub-type Article (original research)
DOI 10.1677/joe.0.1790081
Volume 179
Issue 1
Start page 81
End page 89
Total pages 9
Editor S. Hiller
Place of publication Great Britian
Publisher Society of Endocrinology
Collection year 2003
Language eng
Subject C1
321004 Endocrinology
730105 Endocrine organs and diseases (incl. diabetes)
Abstract Renin and angiotensinogen have been previously found in the rat pancreas, and angiotensin receptors have been located in the apical domain of duct cells. To evaluate the possibility that angiotensin II could be generated within the duct system, we decided to determine whether angiotensinogen is present in rat pancreatic juice and the angiotensinogen-immunoreactive pancreatic cell types that could be responsible for its production. Angiotensinogen was detected in significant amounts by Western blotting in pancreatic juice collected from several individual rats. Different isoforms between plasma and pancreatic juice angiotensinogens were demonstrated by isoelectric focusing. Immunocytochemical experiments revealed angiotensinogen-immunoreactive cells at the periphery of the islets of Langerhans, and confocal microscopy demonstrated that most angiotensinogen-immunoreactive cells were glucagon-secreting cells. Secretion of angiotensinogen did not follow the regulated secretory pathway since it was absent from the glucagon-containing granules. This was confirmed by electron microscopy immunocytochemistry. Duct and acinar cells did not express angiotensinogen at an immunocytochemical detectable level. The present findings indicated an exocrine secretion of angiotensinogen by glucagon-secreting cells and suggest that one of the final targets of the local pancreatic renin-angiotensin system may be the duct epithelium.
Keyword Endocrinology & Metabolism
Ii Receptor Subtypes
Na+-h+ Exchange
Proximal Tubule
Adult-rat
Immunocytochemical Localization
Intestinal Epithelia
Molecular-biology
Canine Pancreas
Binding-sites
Ductal System
Q-Index Code C1

 
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