Storage protein mobilization and thiol protease up-regulation by solid matrix priming in loblolly pine (Pinus taeda) seed embryos

Wu, L., Hallgren, S. W., Huang, Y., Ferris, D. M., Baker, G., Pan, Y., Conway, K. E. and Tauer, C. G. (2003) Storage protein mobilization and thiol protease up-regulation by solid matrix priming in loblolly pine (Pinus taeda) seed embryos. Seed Science and Technology, 31 3: 667-680.

Author Wu, L.
Hallgren, S. W.
Huang, Y.
Ferris, D. M.
Baker, G.
Pan, Y.
Conway, K. E.
Tauer, C. G.
Title Storage protein mobilization and thiol protease up-regulation by solid matrix priming in loblolly pine (Pinus taeda) seed embryos
Journal name Seed Science and Technology   Check publisher's open access policy
ISSN 0251-0952
Publication date 2003
Sub-type Article (original research)
Volume 31
Issue 3
Start page 667
End page 680
Total pages 14
Place of publication Bassersdorf, Switzerland
Publisher Ista-Int Seed Testing Association
Collection year 2003
Language eng
Subject C1
300601 Nutrition and Physiology
620302 Softwood plantations
Abstract Experiments were conducted to investigate physiological mechanisms of solid matrix priming (SMP) on germination enhancement of loblolly pine (Pinus taeda) seeds. During SMP, osmotic potential in the embryo decreased by 0.65 MPa, concentration of crystalloid proteins decreased to 62% and concentrations of buffer soluble proteins and free amino acids increased by 22% and by 166%, respectively. Observations under an electron microscope demonstrated protein bodies in the embryo were mobilized. Inhibitor analysis indicated thiol protease was the dominant enzyme among endopiptidases to degrade the reserved proteins. A fragment of thiol protease was cloned from the primed seed embryos and it has high identities to those thiol proteases responsive to water stress. RNA get blot analysis showed a 1.5 kb thiol protease gene was up-regulated by SMP. Treatment with E64, a thiol protease inhibitor, negated SMP effects on germination performance, water potentials and protein profiles. Based on the experimental results, reserve protein mobilization induced by SMP in the embryo before radicle emergence might be one of the mechanisms to enhance germination in loblolly pine seeds.
Keyword Agronomy
Plant Sciences
Horticulture
6-aminoquinolyl-n-hydroxysuccinimidyl Carbamate
Arabidopsis-thaliana
Cysteine Protease
Germination
Expression
Wheat
Gene
Sequence
Growth
Stress
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 02:24:16 EST