Growth, viral production and metabolism of a Helicoverpa zea cell line in serum-free culture

Lua, L. H. L. and Reid, S. (2003) Growth, viral production and metabolism of a Helicoverpa zea cell line in serum-free culture. Cytotechnology, 42 3: 109-120. doi:10.1023/B:CYTO.0000015795.46813.44


Author Lua, L. H. L.
Reid, S.
Title Growth, viral production and metabolism of a Helicoverpa zea cell line in serum-free culture
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
1573-0778
Publication date 2003
Sub-type Article (original research)
DOI 10.1023/B:CYTO.0000015795.46813.44
Volume 42
Issue 3
Start page 109
End page 120
Total pages 12
Place of publication Dordrecht, Netherlands
Publisher Kluwer Academic Publishng
Collection year 2003
Language eng
Subject C1
290101 Fermentation, Biotechnology and Industrial Microbiology
670602 Crop and animal protection chemicals
Abstract Insect cell cultures have been extensively utilised for means of production for heterologous proteins and biopesticides. Spodoptera frugiperda (Sf9) and Trichoplusia ni (High Five(TM)) cell lines have been widely used for the production of recombinant proteins, thus metabolism of these cell lines have been investigated thoroughly over recent years. The Helicoverpa zea cell line has potential use for the production of a biopesticide, specifically the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV). The growth, virus production, nutrient consumption and waste production of this cell line was investigated under serum-free culture conditions, using SF900II and a low cost medium prototype (LCM). The cell growth ( growth rates and population doubling time) was comparable in SF900II and LCM, however, lower biomass and cell specific virus yields were obtained in LCM. H. zea cells showed a preference for asparagine over glutamine, similar to the High Five(TM) cells. Ammonia was accumulated to significantly high levels (16 mM) in SF900II, which is an asparagine and glutamine rich medium. However, given the absence of asparagine and glutamine in the medium ( LCM), H. zea cells adapted and grew well in the absence of these substrates and no accumulation of ammonia was observed. The adverse effect of ammonia on H. zea cells is unknown since good production of biologically active HaSNPV was achieved in the presence of high ammonia levels. H. zea cells showed a preference for maltose even given an abundance supply of free glucose. Accumulation of lactate was observed in H. zea cell cultures.
Keyword Biotechnology & Applied Microbiology
Cell Biology
Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus
Helicoverpa zea
Serum-free low cost medium
Single-nucleocapsid Nucleopolyhedrovirus
In-vitro Production
Fed-batch Culture
Insect Cells
Armigera Nucleopolyhedrovirus
Serial Passage
Suspension-culture
Free Media
Baculovirus
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 02:20:46 EST