Enrichment of nitrifying microbial communities from shrimp farms and commercial inocula

Paungfoo, C., Prasertsan, P., Intrasungkha, N., Blackall, L. L. and Bhamidimarri, R. (2003) Enrichment of nitrifying microbial communities from shrimp farms and commercial inocula. Water Science and Technology, 48 8: 143-150.

Author Paungfoo, C.
Prasertsan, P.
Intrasungkha, N.
Blackall, L. L.
Bhamidimarri, R.
Title Enrichment of nitrifying microbial communities from shrimp farms and commercial inocula
Journal name Water Science and Technology   Check publisher's open access policy
ISSN 0273-1223
Publication date 2003
Sub-type Article (original research)
Volume 48
Issue 8
Start page 143
End page 150
Total pages 8
Place of publication London, United Kingdom
Publisher IWA Publishing
Collection year 2003
Language eng
Subject C1
270307 Microbial Ecology
770302 Living resources (incl. impacts of fishing on non-target species)
Abstract Nitrifying bacteria were selected from shrimp farm water and sediment (natural seed) in Thailand and from commercial seed cultures. The microbial consortia from each source giving the best ammonia removal during batch culture pre-enrichments were used as inocula for two sequencing batch reactors (SBRs). Nitrifiers were cultivated in the SBRs with 100 mg NH4-N/I and artificial wastewater containing 25 ppt salinity. The two SBRs were operated at a 7 d hydraulic retention time (HRT) for 77 d after which the HRT was reduced to 3.5 d. The amounts of ammonia removed from the influent by microorganisms sourced from the natural seed were 85% and 92% for the 7 d HIRT and the 3.5 d HRT, respectively. The ammonia removals of microbial consortia from the commercial seed were 71% and 83% for these HRTs respectively. The quantity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) was determined in the SBRs using the most probable number (MPN) technique. Both AOB and NOB increased in number over the long-term operation of both SBRs. According to quantitative fluorescence in situ hybridisation (FISH) probing, AOB from the natural seed and commercial seed comprised 21 +/- 2% and 30 +/- 2%, respectively of all bacteria. NOB could not be detected with currently-reported FISH probes, suggesting that novel NOB were enriched from both sources. Taken collectively, the results from this study provide an indication that the nitrifiers from shrimp farm sources are more effective at ammonia removal than those from commercial seed cultures.
Keyword Engineering, Environmental
Environmental Sciences
Water Resources
Ammonia Removal
Effective Microorganisms (em)
Fluorescent In Situ Hybridization (fish)
Nitrifying Bacteria
Sequencing Batch Reactor (sbr)
Shrimp Farm
Targeted Oligonucleotide Probes
Ammonia-oxidizing Bacteria
In-situ Identification
Treatment Plants
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 02:20:19 EST