Molecular analysis of the pathway for the synthesis of thiol tripeptides in the model legume Lotus japonicus

Matamoros, Manuel A., Clemente, Maria R., Sato, Shusei, Asamizu, Erika, Tabata, Satoshi, Ramos, Javier, Moran, Jose F., Stiller, Jiri, Gresshoff, Peter M. and Becana, Manuel (2003) Molecular analysis of the pathway for the synthesis of thiol tripeptides in the model legume Lotus japonicus. Molecular Plant-microbe Interactions, 16 11: 1039-1046. doi:10.1094/MPMI.2003.16.11.1039

Author Matamoros, Manuel A.
Clemente, Maria R.
Sato, Shusei
Asamizu, Erika
Tabata, Satoshi
Ramos, Javier
Moran, Jose F.
Stiller, Jiri
Gresshoff, Peter M.
Becana, Manuel
Title Molecular analysis of the pathway for the synthesis of thiol tripeptides in the model legume Lotus japonicus
Journal name Molecular Plant-microbe Interactions   Check publisher's open access policy
ISSN 0894-0282
Publication date 2003-11
Sub-type Article (original research)
DOI 10.1094/MPMI.2003.16.11.1039
Volume 16
Issue 11
Start page 1039
End page 1046
Total pages 8
Place of publication St. Paul, USA
Publisher American Phytopathological Society
Collection year 2003
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
620108 Grain legumes
Abstract The thiol tripeptides, glutathione (GSH) and homoglutathione (hGSH), perform multiple roles in legumes, including protection against toxicity of free radicals and heavy metals. The three genes involved in the synthesis of GSH and hGSH in the model legume, Lotus japonicus, have been fully characterized and appear to be present as single copies in the genome. The gamma-glutamylcysteine synthetase (gammaecs) gene was mapped on the long arm of chromosome 4 (70.0 centimorgans [cM]) and consists of 15 exons, whereas the glutathione synthetase (gshs) and homoglutathione synthetase (hgshs) genes were mapped on the long arm of chromosome 1 (81.3 cM) and found to be arranged in tandem, with a separation of approximately 8 kb. Both genes consist of 12 exons of exactly the same size (except exon 1, which is similar). Two types of transcripts were detected for the gshs gene, which putatively encode proteins localized in the plastids and cytosol. Promoter regions contain cis-acting regulatory elements that may be involved in the plant's response to light, hormones, and stress. Determination of transcript levels, enzyme activities, and thiol contents in nodules, roots, and leaves revealed that gammaecs and hgshs are expressed in all three plant organs, whereas gshs is significantly functional only in nodules. This strongly suggests an important role of GSH in the rhizobia-legume symbiosis.
Keyword Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Plant Sciences
Gamma-glutamylcysteine Synthetase
Glutathione Synthetase
Homoglutathione Synthetase
Sequence Features
Gsh2 Mutant
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 02:10:38 EST