Comparison of three commercial sparse-matrix crystallization screens

Wooh, J. W., Kidd, R. D., Martin, J. L. and Kobe, B. (2003) Comparison of three commercial sparse-matrix crystallization screens. Acta Crystallographica Section D: Biological Crystallography, 59 4: 769-772. doi:10.1107/S0907444903002919

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Author Wooh, J. W.
Kidd, R. D.
Martin, J. L.
Kobe, B.
Title Comparison of three commercial sparse-matrix crystallization screens
Journal name Acta Crystallographica Section D: Biological Crystallography   Check publisher's open access policy
ISSN 0907-4449
1399-0047
Publication date 2003-04
Sub-type Article (original research)
DOI 10.1107/S0907444903002919
Open Access Status File (Publisher version)
Volume 59
Issue 4
Start page 769
End page 772
Total pages 4
Editor C. E. Bugg
Place of publication Copenhagen, Denmark
Publisher Blackwell Munksgaard
Collection year 2003
Language eng
Subject C1
250503 Characterisation of Macromolecules
780105 Biological sciences
Abstract Sparse-matrix sampling using commercially available crystallization screen kits has become the most popular way of determining the preliminary crystallization conditions for macromolecules. In this study, the efficiency of three commercial screening kits, Crystal Screen and Crystal Screen 2 (Hampton Research), Wizard Screens I and II (Emerald BioStructures) and Personal Structure Screens 1 and 2 (Molecular Dimensions), has been compared using a set of 19 diverse proteins. 18 proteins yielded crystals using at least one crystallization screen. Surprisingly, Crystal Screens and Personal Structure Screens showed dramatically different results, although most of the crystallization formulations are identical as listed by the manufacturers. Higher molecular weight polyethylene glycols and mixed precipitants were found to be the most effective precipitants in this study.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Biophysics
Crystallography
Protein Crystallization
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 02:10:12 EST