Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis

Aimes, RT, Zijlstra, A, Hooper, JD, Ogbourne, SM, Sit, ML, Fuchs, S, Gotley, DC, Quigley, JP and Antalis, TM (2003) Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thrombosis And Haemostasis, 89 3: 561-572.

Author Aimes, RT
Zijlstra, A
Hooper, JD
Ogbourne, SM
Sit, ML
Fuchs, S
Gotley, DC
Quigley, JP
Antalis, TM
Title Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis
Journal name Thrombosis And Haemostasis   Check publisher's open access policy
ISSN 0340-6245
Publication date 2003
Sub-type Article (original research)
Volume 89
Issue 3
Start page 561
End page 572
Total pages 12
Editor Klaus T Preissner
Place of publication Germany
Publisher Schattauer GmbH
Collection year 2003
Language eng
Subject C1
321008 Haematology
730108 Cancer and related disorders
Abstract Many serine proteases play important regulatory roles in complex biological systems, but only a few have been linked directly with capillary morphogenesis and angiogenesis. Here we provide evidence that serine protease activities, independent of the plasminogen activation cascade, are required for microvascular endothelial cell reorganization and capillary morphogenesis in vitro. A homology cloning approach targeting conserved motifs present in all serine proteases, was used to identify candidate serine proteases involved in these processes, and revealed 5 genes (acrosin, testisin, neurosin, PSP and neurotrypsin), none of which had been associated previously with expression in endothelial cells. A subsequent gene-specific RT-PCR screen for 22 serine proteases confirmed expression of these 5 genes and identified 7 additional serine protease genes expressed by human endothelial cells, urokinase-type plasminogen activator, protein C,TMPRSS2, hepsin, matriptase/ MT-SPI, dipepticlylpepticlase IV, and seprase. Differences in serine protease gene expression between microvascular and human umbilical vein endothelial cells (HUVECs) were identified and several serine protease genes were found to be regulated by the nature of the substratum, ie. artificial basement membrane or fibrillar type I collagen. mRNA transcripts of several serine protease genes were associated with blood vessels in vivo by in situ hybridization of human tissue specimens. These data suggest a potential role for serine proteases, not previously associated with endothelium, in vascular function and angiogenesis.
Keyword Hematology
Peripheral Vascular Disease
Serine Protease
Endothelial Cell
Tubule Morphogenesis
Tissue-specific Expression
Capillary-like Structures
Cdna Sequence
Zyme/protease M/neurosin
Transmembrane Domain
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Medicine Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 64 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 02:02:04 EST