A protective effect of early pregnancy factor on experimental autoimmune encephalomyelitis induced in Lewis rats by inoculation with myelin basic protein

Harness, J, Cavanagh, A, Morton, H and McCombe, P (2003) A protective effect of early pregnancy factor on experimental autoimmune encephalomyelitis induced in Lewis rats by inoculation with myelin basic protein. Journal of The Neurological Sciences, 216 1: 33-41. doi:10.1016/S0022-510X(03)00212-0


Author Harness, J
Cavanagh, A
Morton, H
McCombe, P
Title A protective effect of early pregnancy factor on experimental autoimmune encephalomyelitis induced in Lewis rats by inoculation with myelin basic protein
Journal name Journal of The Neurological Sciences   Check publisher's open access policy
ISSN 0022-510X
Publication date 2003-12-15
Sub-type Article (original research)
DOI 10.1016/S0022-510X(03)00212-0
Volume 216
Issue 1
Start page 33
End page 41
Total pages 9
Editor R. A. Lewis
R. P. Lisak
Place of publication Amsterdam
Publisher Elsevier BV
Collection year 2003
Language eng
Subject C1
320207 Autoimmunity
730102 Immune system and allergy
Abstract Experimental antoimmune encephalomyelitis (EAE) is an organ-specific autoimmune disease characterised by inflammation and demyelination of the central nervous system and is the best available animal model of multiple sclerosis (MS). Since previous studies have shown that EAE is less severe or is delayed in onset during pregnancy and that administration of the pregnancy hormone early pregnancy factor (EPF) down-regulates EAE, experiments in the present study were designed to explore further the role of EPF in EAE. By using the rosette inhibition test, the standard bioassay for EPF and, by semi-quantitative RT-PCR techniques, we have now shown that inflammatory cells from the spinal cord of rats with EAE can produce and secrete EPF, with production being greatest during recovery from disease. Administration of EPF to rats with EAE resulted in a significant increase in the expression of IL-4 and IL-10 mRNA and a significant decrease in IFN-gamma mRNA expression in spinal cord inflammatory cells. Encephalitogenic MBP-specific T cell lines were prepared from popliteal lymph nodes of rats with EAE. Proliferation assays using these cells demonstrated the ability of exogenous EPF to down-regulate the responses of T lymphocytes to MBP. (C) 2003 Elsevier B.V. All rights reserved.
Keyword Clinical Neurology
Neurosciences
Experimental Autoimmune Encephalomyelitis
Multiple Sclerosis
Early Pregnancy Factor
Heat Shock Protein
Chaperonin 10
Reproductive Immunology
Pregnancy
Cytokines
Experimental Allergic Encephalomyelitis
Central-nervous-system
T-cell Receptors
Spinal-cord
Embryonic-development
Multiple-sclerosis
Monoclonal-antibodies
V-beta-8.2(+) Cells
Cytokine Expression
Messenger-rna
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 01:51:26 EST