Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing

Broderick, D., Idaghdour, Y., Korrida, A. and Hellmich, J. (2003) Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing. Conservation Genetics, 4 6: 793-800. doi:10.1023/B:COGE.0000006111.65204.c9


Author Broderick, D.
Idaghdour, Y.
Korrida, A.
Hellmich, J.
Title Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing
Journal name Conservation Genetics   Check publisher's open access policy
ISSN 1566-0621
Publication date 2003-11
Sub-type Article (original research)
DOI 10.1023/B:COGE.0000006111.65204.c9
Volume 4
Issue 6
Start page 793
End page 800
Total pages 8
Place of publication Dordrecht, The Netherlands
Publisher Kluwer Academic Publishing
Collection year 2003
Language eng
Subject C1
270203 Population and Ecological Genetics
780105 Biological sciences
Abstract Recent advances in molecular biology have made it possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. Here we use faeces as a source of DNA and mtDNA sequence data to elucidate the relationship among Spanish and Moroccan populations of great bustards. 834 bp of combined control region and cytochrome-b mtDNA fragments revealed four variable sites that defined seven closely related haplotypes in 54 individuals. Morocco was fixed for a single mtDNA haplotype that occurs at moderate frequency (28%) in Spain. We could not differentiate among the sampled Spanish populations of Caceres and Andalucia but these combined populations were differentiated from the Moroccan population. Estimates of gene flow (Nm = 0.82) are consistent with extensive observations on the southern Iberian peninsular indicating that few individuals fly across the Strait of Gibraltar. We demonstrate that both this sea barrier and mountain barriers in Spain limit dispersal among adjacent great bustard populations to a similar extent. The Moroccan population is of high ornithological significance as it holds the only population of great bustards in Africa. This population is critically small and genetic and observational data indicate that it is unlikely to be recolonised via immigration from Spain should it be extirpated. In light of the evidence presented here it deserves the maximum level of protection.
Keyword Biodiversity Conservation
Genetics & Heredity
Faeces
Gene Flow
Mtdna
Non-invasive Genetics
Otis Tarda
Otis-tarda
Mitochondrial
Spain
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Wed, 15 Aug 2007, 01:46:42 EST