Distribution of Sugarcane mosaic virus in sugarcane plants

Putra, LK, Ogle, HJ, James, AP and Whittle, PJL (2003) Distribution of Sugarcane mosaic virus in sugarcane plants. Australasian Plant Pathology, 32 2: 305-307. doi:10.1071/AP03011


Author Putra, LK
Ogle, HJ
James, AP
Whittle, PJL
Title Distribution of Sugarcane mosaic virus in sugarcane plants
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
Publication date 2003
Sub-type Article (original research)
DOI 10.1071/AP03011
Volume 32
Issue 2
Start page 305
End page 307
Total pages 3
Editor E.J. Cother
Place of publication Melbourne, Victoria
Publisher CSIRO
Collection year 2003
Language eng
Subject C1
300204 Plant Protection (Pests, Diseases and Weeds)
620106 Sugar
Abstract Variation in the concentration of virus in different parts of the plant has implications for virus-indexing programs. To allow more reliable detection of Sugarcane mosaic virus (SCMV), the distribution of the virus in sugarcane plants after artificial inoculation was studied using a reverse transcription polymerase chain reaction (RT-PCR) assay. Leaves of susceptible and moderately resistant sugarcane were mechanically inoculated with SCMV 6 weeks after planting. Weekly for 8 weeks after inoculation, plants were examined for mosaic symptoms and samples of leaves, roots and tillers were tested by RT-PCR to detect virus. SCMV moved from the point of inoculation to younger leaves, roots and tillers and eventually to leaves that emerged prior to inoculation. The pattern of SCMV distribution in moderately resistant and susceptible cultivars was not substantially different. However, the virus moved more slowly in the moderately resistant than in the susceptible cultivar. Young leaves proved to be the most suitable tissue for testing.
Keyword Plant Sciences
Protocol
Tissue
Pcr
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Agriculture and Food Sciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 01:42:29 EST