Burkholderia pseudomallei: Another emerging pathogen in cystic fibrosis

O'Carroll, M. R., Kidd, T. J., Coulter, C., Smith, H., Rose, H., Harbour, C. and Bell, S. C. (2003). Burkholderia pseudomallei: Another emerging pathogen in cystic fibrosis. In: P. J. Thompson, TSANZ Oral Abstracts. TSANZ ASM 2003, Adelaide, SA, (A47-A47). 4 – 9 April 2003. doi:10.1111/j.1440-1843.2003.supp_1.x

Author O'Carroll, M. R.
Kidd, T. J.
Coulter, C.
Smith, H.
Rose, H.
Harbour, C.
Bell, S. C.
Title of paper Burkholderia pseudomallei: Another emerging pathogen in cystic fibrosis
Conference name TSANZ ASM 2003
Conference location Adelaide, SA
Conference dates 4 – 9 April 2003
Proceedings title TSANZ Oral Abstracts   Check publisher's open access policy
Place of Publication Carlton South, Australia
Publisher Blackwell Publishing Asia
Publication Year 2003
DOI 10.1111/j.1440-1843.2003.supp_1.x
ISSN 1323-7799
Editor P. J. Thompson
Volume 8
Issue Suppl 2
Start page A47
End page A47
Total pages 1
Language eng
Formatted Abstract/Summary
Background: B. pseudomallei, an endemic gram-negative bacterium found in soil and water in northern Australia and South-east Asia, is an important cause of acute fulminant pneumonia and septicaemia. Subacute and chronic forms of the disease also occur. Two case reports of adults with CF, who became infected with B. pseudomallei shortly after extended vacations in Thailand, have recently been reported.

Methods: The clinical course, molecular characteristics, serology and response to therapy are described in four patients with CF. Molecular confirmation of B. pseudomallei was obtained using a 16S rRNA PCR and B. cepacia complex was excluded using RecA PCR. Genotyping was performed using both randomly amplified polymorphic DNA (RAPD) PCR and pulse field gel electrophoresis (PFGE) following digestion with SpeI.

Results: Four patients are described (3 male, mean FEV1 66% predicted (range 31–100%), mean duration of infection 32 months (range 2–96)). All but one patient lived in tropical Queensland. Two patients (with the longest duration of infection) deteriorated clinically. Both responded to intravenous treatment specifically targeting B. pseudomallei. Each isolate was B. pseudomallei 16S rRNA PCR positive and RecA PCR negative. Multiple isolates were available for genotypic analysis (range 2–11 per patient). PFGE of a sample isolate from each patient revealed strains to be unique. RAPD analysis showed retention of the same strain within an individual over time.

Conclusions: B. pseudomallei can cause chronic infection in CF patients living or visiting endemic regions. These patients suggest that B. pseudomallei is a pathogen in CF. Identical isolates are retained over time and are unique, consistent with likely environmental acquisition. Laboratories with expertise in CF microbiology should not confuse B. pseudomallei with B. cepacia.
Subjects CX
321027 Respiratory Diseases
730110 Respiratory system and diseases (incl. asthma)
Q-Index Code CX
Additional Notes Respiratory Nurses SIG Oral Presentations: P 123

Document type: Conference Paper
Collection: School of Chemistry and Molecular Biosciences
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Created: Wed, 15 Aug 2007, 01:40:52 EST