Nitrifying microbial community analysis of nitrite accumulating biofilm reactor by fluorescence in situ hybridization

Han, D.W., Chang, J.S. and Kim, D (2003) Nitrifying microbial community analysis of nitrite accumulating biofilm reactor by fluorescence in situ hybridization. Water Science and Technology, 47 1: 97-104.


Author Han, D.W.
Chang, J.S.
Kim, D
Title Nitrifying microbial community analysis of nitrite accumulating biofilm reactor by fluorescence in situ hybridization
Journal name Water Science and Technology   Check publisher's open access policy
ISSN 0273-1223
Publication date 2003
Sub-type Article (original research)
Volume 47
Issue 1
Start page 97
End page 104
Total pages 8
Editor P. Wilderer
Place of publication United Kingdom
Publisher IWA Publishing
Collection year 2003
Language eng
Subject C1
290699 Chemical Engineering not elsewhere classified
779999 Other
0904 Chemical Engineering
Abstract Biological nitrogen removal via nitrite pathway in wastewater treatment is very important especially in the cost of aeration and as an electron donor for denitrification. Wastewater nitrification and nitrite accumulations were carried out in a biofilm reactor. The biofilm reactor showed almost complete nitrification and most of the oxidized ammonium was present as nitrite at the ammonium load of 1.2 kg N/m3/d. Nitrite accumulation was achieved by the selective inhibition of nitrite oxidizers by free ammonia and oxygen limitation. Nitrite oxidation activity was recovered as soon as the inhibition factor was removed. Fluorescence in situ hybridization studies of the nitrite accumulating biofilm system have shown that genus Nitrosomonas which is specifically hybridized with probe NSM 156 was the dominant nitrifying bacteria while Nitrospira was less abundant than those of normal nitrification systems. Further FISH analysis showed that the combinations of Nitrosomonas and Nitrospira cells were identified as important populations of nitrifying bacteria in an autotrophic nitrifying biofilm system.
Keyword fish
Inhibition
Nitrification
Nitrite accumulation
Nitrospira
Nitrosomonas
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
Advanced Water Management Centre Publications
 
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Created: Wed, 15 Aug 2007, 01:40:09 EST