Identification and characterization of the hepatic stellate cell transferrin receptor

Bridle, Kim R., Crawford, Darrell H. G. and Ramm, Grant A. (2003) Identification and characterization of the hepatic stellate cell transferrin receptor. American Journal of Pathology, 162 5: 1661-1667. doi:10.1016/S0002-9440(10)64300-3


Author Bridle, Kim R.
Crawford, Darrell H. G.
Ramm, Grant A.
Title Identification and characterization of the hepatic stellate cell transferrin receptor
Journal name American Journal of Pathology   Check publisher's open access policy
ISSN 0002-9440
Publication date 2003-05
Sub-type Article (original research)
DOI 10.1016/S0002-9440(10)64300-3
Volume 162
Issue 5
Start page 1661
End page 1667
Total pages 7
Editor Nelson Fausto
Place of publication USA
Publisher American Society for Investigative Pathology
Collection year 2003
Language eng
Subject C1
321006 Gastroenterology and Hepatology
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract Activated hepatic stellate cells have been implicated in the fibrogenic process associated with iron overload, both in animal models and in human hemochromatosis. Previous studies have evaluated the role of ferritin/ferritin receptor interactions in the activation of stellate cells and subsequent fibrogenesis; however, the role of transferrin in hepatic stellate cell biology is unknown. This study was designed to identify and characterize the stellate cell transferrin receptor and to evaluate the influence of transferrin on stellate cell activation. Identification and characterization of the stellate cell transferrin receptor was determined by competitive displacement assays. The effect of transferrin on stellate cell activation was assessed using western blot analysis for alpha-smooth muscle actin expression, [H-3]Thymidine incorporation, and real-time RT-PCR for procollagen 1(I) mRNA expression. A specific receptor for rat transferrin was observed on activated but not quiescent stellate cells. Transferrin significantly increased the expression of alpha-smooth muscle actin, but caused a decrease in proliferation. Transferrin induced a significant increase in procollagen alpha1(I) mRNA expression. In conclusion, this study has demonstrated for the first time a specific, high affinity receptor for rat transferrin on activated hepatic stellate cells, which via interaction with transferrin regulates stellate cell activation. This suggests that transferrin may be an important factor in the activation of hepatic stellate cells in conditions of iron overload.
Keyword Pathology
Iron Uptake
Rat Hepatocytes
Kupffer Cells
Growth-factor
Proliferation
Culture
Binding
Reticulocytes
Activation
Metabolism
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 01:36:59 EST