Characterization of Treponema phagedenis-like spirochetes isolated from papillomatous digital dermatitis lesions in dairy cattle

Trott, D. J., Moeller, M. R., Zuerner, R. L., Goff, J. P., Waters, W. R., Alt, D. P., Walker, R. L. and Wannemuehler, M. J. (2003) Characterization of Treponema phagedenis-like spirochetes isolated from papillomatous digital dermatitis lesions in dairy cattle. Journal of Clinical Microbiology, 41 6: 2522-2529. doi:10.1128/JCM.41.6.2522-2529.2003

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Author Trott, D. J.
Moeller, M. R.
Zuerner, R. L.
Goff, J. P.
Waters, W. R.
Alt, D. P.
Walker, R. L.
Wannemuehler, M. J.
Title Characterization of Treponema phagedenis-like spirochetes isolated from papillomatous digital dermatitis lesions in dairy cattle
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2003
Sub-type Article (original research)
DOI 10.1128/JCM.41.6.2522-2529.2003
Open Access Status File (Publisher version)
Volume 41
Issue 6
Start page 2522
End page 2529
Total pages 8
Editor R. C. Tikton
A. B. Orderdonk
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2003
Language eng
Abstract Four spirochete strains were isolated from papillomatous digital dermatitis (PDD) lesions in Iowa dairy cattle and compared with two previously described spirochete strains isolated from dairy cattle in California. These six strains shared an identical 16S ribosomal DNA sequence that was 98% similar to Treponema phagedenis and 99% similar to the uncultivated PDD spirochete sequence DDLK-4. The whole-cell protein profiles resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these six strains were similar. However, these strains showed differences in the antigenic diversity of lipopolysaccharide (LPS). Genetic diversity was also detected by pulsed-field gel electrophoresis of genomic DNA digests, revealing differences among five of the six strains. Serum immunoglobulin G antibodies from dairy cattle with active PDD lesions reacted with the LPS of all but one PDD spirochete strain. Likewise, peripheral blood mononuclear cells from cattle with active PDD lesions produced blastogenic responses to one of the two California isolates. Both antibody and lymphocyte blastogenic responses were reduced in convalescent dairy cattle, suggesting the immune response to these spirochetes has short duration. These results demonstrate genetic and antigenic diversity among T. phagedenis-like treponemes and provide further evidence for the involvement of these spirochetes in the pathogenesis of PDD.
Keyword Treponema Phagedenis
Siprochetes
Dairy Cattle
Microbiology
Serpulina-pilosicoli
Molecular Epidemiology
Human Periodontitis
Risk-factors
Hyodysenteriae
Lipopolysaccharide
Infections
Leptospira
Membrane
Disease
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 01:35:33 EST