Dietary interactions influence the effects of bovine folate-binding protein on the bioavailability of tetrahydrofolates in rats

Jones, ML, Treloar, T and Nixon, PF (2003) Dietary interactions influence the effects of bovine folate-binding protein on the bioavailability of tetrahydrofolates in rats. Journal of Nutrition, 133 2: 489-495.

Author Jones, ML
Treloar, T
Nixon, PF
Title Dietary interactions influence the effects of bovine folate-binding protein on the bioavailability of tetrahydrofolates in rats
Journal name Journal of Nutrition   Check publisher's open access policy
ISSN 0022-3166
Publication date 2003
Sub-type Article (original research)
Volume 133
Issue 2
Start page 489
End page 495
Total pages 7
Place of publication USA
Publisher American Society for Nutritional Sciences
Collection year 2003
Language eng
Subject C1
270199 Biochemistry and Cell Biology not elsewhere classified
321205 Nutrition and Dietetics
730215 Nutrition
Abstract The newborns of mammals have a high folate demand, yet obtain adequate folate nutrition solely from their mothers' milk despite its low folate content. Milk folate is entirely bound by an excess of folate-binding protein (FBP), prompting speculation that FBP may affect the bioavailability of the limited folate supply. Previous research has shown that FBP-bound folic acid is more gradually absorbed, thereby reducing the peak plasma folate concentration and preventing loss into the urine. Natural folates are reduced derivatives of folic acid, with milk predominantly containing 5-methyltetrahydrofolate, yet little research has been carried out to determine the role of FBP in the bioavailability of reduced folates. We studied the effect of FBP on folate nutrition of rats in both single-dose and 4-wk feeding experiments. The effect of FBP was influenced by the presence of other milk components. FBP increased bioavailability of dietary folate when it was consumed with other whey proteins or with soluble casein. However, in the presence of acid-precipitated casein or a whey preparation enriched in lipids, bioavailability was decreased. These results highlight the difficulties of extrapolating from experimental results obtained using purified diets alone and of studying interactions among dietary components. They suggest that the addition of FBP-rich foods to folate-rich foods could enhance the bioavailability of natural folates, but that the outcome of such a combination would depend on interactions with other components of the diet.
Keyword Nutrition & Dietetics
Folate-binding Protein
5-formyltetrahydrofolate
5-methyltetrahydrofolate
Bioavailability
Dietary Interactions
Amino-acid-sequence
Folic-acid
Cows Milk
Affinity-chromatography
Receptor-alpha
Kb Cells
Whey
Beta
Purification
Absorption
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 01:34:13 EST