Contextual binding of p120ctn to E-cadherin at the basolateral plasma membrane in polarized epithelia

Miranda, K. C., Joseph, S. R., Yap, A. S., Teasdale, R. D. and Stow, J. L. (2003) Contextual binding of p120ctn to E-cadherin at the basolateral plasma membrane in polarized epithelia. Journal of Biological Chemistry, 278 44: 43480-43488. doi:10.1074/jbc.M305525200

Author Miranda, K. C.
Joseph, S. R.
Yap, A. S.
Teasdale, R. D.
Stow, J. L.
Title Contextual binding of p120ctn to E-cadherin at the basolateral plasma membrane in polarized epithelia
Formatted title
Contextual Binding of p120ctn to E-cadherin at the Basolateral Plasma Membrane in Polarized Epithelia
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2003
Sub-type Article (original research)
DOI 10.1074/jbc.M305525200
Volume 278
Issue 44
Start page 43480
End page 43488
Total pages 9
Editor Herbert Tabor
Place of publication Bethesda, USA
Publisher American society for Biochemistry and Molecular Biology
Collection year 2003
Language eng
Subject C1
270103 Protein Targeting and Signal Transduction
780106 Political science and public policy
Abstract E-cadherin-catenin complexes mediate cell-cell adhesion on the basolateral membrane of epithelial cells. The cytoplasmic tail of E-cadherin supports multiple protein interactions, including binding of beta-catenin at the C terminus and of p120(ctn) to the juxtamembrane domain. The temporal assembly and polarized trafficking of the complex or its individual components to the basolateral membrane are not fully understood. In Madin-Darby canine kidney cells at steady state and after treatment with cycloheximide or temperature blocks, E-cadherin and beta-catenin localized to the Golgi complex, but p120ctn was found only at the basolateral plasma membrane. We previously identified a dileucine sorting motif (Leu(586)-Leu(587), termed S1) in the juxtamembrane domain of E-cadherin and now show that it is required to target full-length E-cadherin to the basolateral membrane. Removal of S1 resulted in missorting of E-cadherin mutants (EcadDeltaS1) to the apical membrane; beta-catenin was simultaneously missorted and appeared at the apical membrane. p120(ctn) was not mistargeted with EcadDeltaS1, but could be recruited to the E-cadherin-catenin complex only at the basolateral membrane. These findings help define the temporal assembly and sorting of the E-cadherin-catenin complex and show that membrane recruitment of p120(ctn) in polarized cells is contextual and confined to the basolateral membrane.
Keyword Biochemistry & Molecular Biology
Darby Canine Kidney
Di-leucine Motif
P120 Catenin
Mdck Cells
Cytoplasmic Tail
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 01:33:24 EST