Matrix metalloproteinase-2 and-9 involvement in canine tumors

Loukopoulos, P., Mungall, B.A., Straw, R.C., Thornton, J.R. and Robinson, W.F. (2003) Matrix metalloproteinase-2 and-9 involvement in canine tumors. Veterinary Pathology, 40 4: 382-394. doi:10.1354/vp.40-4-382

Author Loukopoulos, P.
Mungall, B.A.
Straw, R.C.
Thornton, J.R.
Robinson, W.F.
Title Matrix metalloproteinase-2 and-9 involvement in canine tumors
Journal name Veterinary Pathology   Check publisher's open access policy
ISSN 0300-9859: 1544-2217
Publication date 2003-07
Sub-type Article (original research)
DOI 10.1354/vp.40-4-382
Open Access Status DOI
Volume 40
Issue 4
Start page 382
End page 394
Total pages 13
Editor D.F. Kusewitt
Place of publication Lawrence, Kansas, U.S.A.
Publisher American College of Veterinary Pathologists
Collection year 2003
Language eng
Subject C1
279999 Biological Sciences not elsewhere classified
780105 Biological sciences
Abstract Matrix metalloproteinases (MMPs) are a family of enzymes implicated in the degradation and remodeling of extracellular matrix and in vascularization. They are also involved in pathologic processes such as tumor invasion and metastasis in experimental cancer models and in human malignancies. We used gelatin zymography and inummohistochemistry to determine whether MMP-2 and MMP-9 are present in canine tumors and normal tissues and whether MMP production correlates with clinicopathologic parameters of prognostic importance. High levels of pro-MMP-9, pro-MMP-2, and active MMP-2 were detected in most canine tumors. Significantly higher MMP levels were measured in canine tumors than in nontumors, malignancies had higher MMP levels than benign tumors, and sarcomas had higher active MMP-2 than carcinomas. Cartilaginous tumors produced higher MMP levels than did nonsarcomatous malignancies, benign tumors, and normal tissues, and significantly greater MMP-2 than osteosarcomas and fibrosarcomas. Pro-MMP-9 production correlated with the histologic grade of osteosarcomas. The 62-kd form of active MMP-2 was detected only in high-grade, p53-positive, metastatic malignancies. Zymography proved to be a sensitive and quantitative technique for the assessment of MMP presence but has the limitation of requiring fresh tissue; inummohistochemistry is qualitative and comparatively insensitive but could be of value in archival studies. MMP presence was shown in a range of canine tumors, and their link to tumor type and grade was demonstrated for the first time. This study will allow a substantially improved evaluation of veterinary cancer patients and provides baseline information necessary for the design of clinical trials targeting MMPs.
Keyword Metalloproteinase-2
Veterinary Sciences
Gelatin Zymography
Matrix Metalloproteinases
Tissue Inhibitor
Iv Collagenase
Increased Expression
Equine Laminitis
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2004 Higher Education Research Data Collection
School of Veterinary Science Publications
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Citation counts: TR Web of Science Citation Count  Cited 39 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 42 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 01:27:40 EST