Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein

Suzuki, A, Kusakai, G, Kishimoto, A, Lu, J, Ogura, T, Lavin, MF and Esumi, H (2003) Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein. Journal of Biological Chemistry, 278 1: 48-53. doi:10.1074/jbc.M206025200

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Author Suzuki, A
Kusakai, G
Kishimoto, A
Lu, J
Ogura, T
Lavin, MF
Esumi, H
Title Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2003-01-01
Sub-type Article (original research)
DOI 10.1074/jbc.M206025200
Open Access Status File (Publisher version)
Volume 278
Issue 1
Start page 48
End page 53
Total pages 6
Editor H. Tabor
Place of publication Bethesda, U.S.A.
Publisher American Society for Biochemistry & Molecular Biology
Collection year 2003
Language eng
Subject C1
320305 Medical Biochemistry - Proteins and Peptides
730107 Inherited diseases (incl. gene therapy)
Abstract We identified a novel human AMP-activated protein kinase (AMPK) family member, designated ARK5, encoding 661 amino acids with an estimated molecular mass of 74 kDa. The putative amino acid sequence reveals 47, 45.8, 42.4, and 55% homology to AMPK-alpha1, AMPK-alpha2, MELK and SNARE respectively, suggesting that it is a new member of the AMPK family. It has a putative Akt phosphorylation motif at amino acids 595600, and Ser(600) was found to be phosphorylated by active Akt resulting in the activation of kinase activity toward the SAMS peptide, a consensus AMPK substrate. During nutrient starvation, ARK5 supported the survival of cells in an Akt-dependent manner. In addition, we also demonstrated that ARK5, when activated by Akt, phosphorylated the ATM protein that is mutated in the human genetic disorder ataxia-telangiectasia and also induced the phosphorylation of p53. On the basis of our current findings, we propose that a novel AMPK family member, ARK5, is the tumor cell survival factor activated by Akt and acts as an ATM kinase under the conditions of nutrient starvation.
Keyword Biochemistry & Molecular Biology
Cell-cycle Checkpoint
Ataxia-telangiectasia
Phosphorylation
Activation
Subfamily
Cloning
Gene
P53
Pathways
Damage
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Medicine Publications
 
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