Detection of West Nile Virus Infection in birds in the United States by blocking ELISA and immunohistochemistry

Jozan, M., Evans, R., McLean, R., Hall, R., Tangredi, B., Reed, L. and Scott, J. (2003) Detection of West Nile Virus Infection in birds in the United States by blocking ELISA and immunohistochemistry. Vector-borne And Zoonotic Diseases, 3 3: 99-110. doi:10.1089/153036603768395799

Author Jozan, M.
Evans, R.
McLean, R.
Hall, R.
Tangredi, B.
Reed, L.
Scott, J.
Title Detection of West Nile Virus Infection in birds in the United States by blocking ELISA and immunohistochemistry
Journal name Vector-borne And Zoonotic Diseases   Check publisher's open access policy
ISSN 1530-3667
Publication date 2003
Sub-type Article (original research)
DOI 10.1089/153036603768395799
Volume 3
Issue 3
Start page 99
End page 110
Total pages 12
Place of publication Larchmont
Publisher Mary Ann Liebert, Inc
Collection year 2003
Language eng
Subject C1
270303 Virology
270304 Infectious Agents
300506 Pathology
730305 Diagnostic methods
730212 Disease distribution and transmission
730101 Infectious diseases
Abstract A blocking ELISA targeting an immunodominant West Nile epitope on the West Nile Virus NS1 protein was assessed for the detection of West Nile-specific antibodies in blood samples collected from 584 sentinel chickens and 238 wild birds collected in-New Jersey from May-December 2000. Ten mallard ducks (Anas platyrhynchos) experimentally infected with West Nile virus and six uninfected controls were also tested. The ELISA proved specific in detecting WNV antibodies in 9/10 chickens and 4/4 wild birds previously confirmed as positive by Plaque Reduction Neutralization test (PRNT) at the Center for Disease Control, Division of Vector Borne Diseases, Fort Collins, CO, USA (CDC). Nine out of the ten experimentally infected mallard ducks also tested positive for WN antibodies in the blocking ELISA, while 6/6 uninfected controls did not. Additionally, 1705 wild birds, collected in New Jersey from December 2000-November 2001 and Long Island, New York between November 1999 and August 2001 were also tested for WN antibodies by the blocking ELISA. These tests identified 30 positive specimens, 12 of which had formalin-fixed tissues available to allow detection of WN specific viral antigen in various tissues by WNV-specific immunohistochemistry. Our results indicate that rapid and specific detection of antibodies to WN virus in sera from a range of avian species by blocking ELISA is an effective strategy for WN Virus surveillance in avian hosts. In combination with detection of WN-specific antigens in tissues by immunohistochemistry (IHC) the blocking ELISA will also be useful for confirming WN infection in diseased birds.
Keyword Public, Environmental & Occupational Health
Infectious Diseases
West Nile Virus
Blocking Elisa
Wild Birds
Serum-blood Spot
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 01:23:56 EST