Complete genomic sequence of the Australian south-west genotype of Sindbis virus: Comparisons with other Sindbis strains and identification of a unique deletion in the 3 '-untranslated region

Saleh, S. M., Poidinger, M., Mackenzie, J. S., Broom, A. K., Lindsay, M. D. and Hall, R. A. (2003) Complete genomic sequence of the Australian south-west genotype of Sindbis virus: Comparisons with other Sindbis strains and identification of a unique deletion in the 3 '-untranslated region. Virus Genes, 26 3: 317-327. doi:10.1023/A:1024407516352


Author Saleh, S. M.
Poidinger, M.
Mackenzie, J. S.
Broom, A. K.
Lindsay, M. D.
Hall, R. A.
Title Complete genomic sequence of the Australian south-west genotype of Sindbis virus: Comparisons with other Sindbis strains and identification of a unique deletion in the 3 '-untranslated region
Journal name Virus Genes   Check publisher's open access policy
ISSN 0920-8569
Publication date 2003
Sub-type Article (original research)
DOI 10.1023/A:1024407516352
Volume 26
Issue 3
Start page 317
End page 327
Total pages 11
Place of publication The Netherlands
Publisher Kluwer Academic
Collection year 2003
Language eng
Subject C1
270303 Virology
270208 Molecular Evolution
780105 Biological sciences
730212 Disease distribution and transmission
Abstract Our previous studies have shown that two distinct genotypes of Sindbis (SIN) virus occur in Australia. One of these, the Oriental/Australian type, circulates throughout most of the Australian continent, whereas the recently identified south-west (SW) genetic type appears to be restricted to a distinct geographic region located in the temperate south-west of Australia. We have now determined the complete nucleotide and translated amino acid sequences of a SW isolate of SIN virus (SW6562) and performed comparative analyses with other SIN viruses at the genomic level. The genome of SW6562 is 11,569 nucleotides in length, excluding the cap nucleotide and poly (A) tail. Overall this virus differs from the prototype SIN virus (strain AR339) by 23% in nucleotide sequence and 12.5% in amino acid sequence. Partial sequences of four regions of the genome of four SW isolates were determined and compared with the corresponding sequences from a number of SIN isolates from different regions of the World. These regions are the non-structural protein (nsP3), the E2 gene, the capsid gene, and the repeated sequence elements (RSE) of the 3'UTR. These comparisons revealed that the SW SIN viruses were more closely related to South African and European strains than to other Australian isolates of SIN virus. Thus the SW genotype of SIN virus may have been introduced into this region of Australia by viremic humans or migratory birds and subsequently evolved independently in the region. The sequence data also revealed that the SW genotype contains a unique deletion in the RSE of the 3'UTR region of the genome. Previous studies have shown that deletions in this region of the SIN genome can have significant effects on virus replication in mosquito and avian cells, which may explain the restricted distribution of this genotype of SIN virus.
Keyword Genetics & Heredity
Virology
Alphavirus
Nucleotide Sequence
Repeat Sequence Elements
Sindbis Virus
Complete Nucleotide-sequence
Ross River Virus
Rna
Replication
Evolution
Agent
Q-Index Code C1

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 19:39:17 EST