Localization of the Epstein-Barr virus protein LMP 1 to exosomes

Flanagan, James, Middeldorp, Jaap and Sculley, Tom (2003) Localization of the Epstein-Barr virus protein LMP 1 to exosomes. Journal of General Virology, 84 7: 1871-1879. doi:10.1099/vir.0.18944-0


Author Flanagan, James
Middeldorp, Jaap
Sculley, Tom
Title Localization of the Epstein-Barr virus protein LMP 1 to exosomes
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2003
Sub-type Article (original research)
DOI 10.1099/vir.0.18944-0
Volume 84
Issue 7
Start page 1871
End page 1879
Total pages 9
Editor G. L. Smith
Place of publication Basingstoke, U.K.
Publisher Society for General Microbiology
Collection year 2003
Language eng
Subject C1
320202 Cellular Immunology
730108 Cancer and related disorders
Abstract The Epstein-Barr virus latent membrane protein (LMP 1) functions as a constitutively active signalling molecule and associates in lipid rafts clustered with other signalling molecules. Using immunofluorescent confocal microscopy, LMP 1 was shown to have an heterogeneous distribution among individual cells which was not related to the cell cycle stage. LMP 1 was shown to localize to intracellular compartments in cells other than the plasma membrane, Co-labelling of cells with both an LIMP 1 antibody and an antibody to the Golgi protein GS15 revealed that the intracellular LMP 1 partly co-localized with the Golgi apparatus. Further confirmation of intracellular LMP 1 localization was obtained by immunoelectron microscopy with rabbit polyclonal LIMP 1 antibodies and cryosectioning. As well as being present in intracellular foci, LMP 1 co-localized in part with MHC-II and was present on exosomes derived from a lymphoblastoid cell line. Preparations of LMP 1 containing exosomes were shown to inhibit the proliferation of peripheral blood mononuclear cells, suggesting that LIMP 1 could be involved in immune regulation. This may be of particular relevance in EBV-associated tumours such as nasopharyngeal carcinoma and Hodgkin's disease, as LMP 1-containing exosomes may be taken up by infiltrating T-lymphocytes, where LMP 1 could exert an anti-proliferative effect, allowing the tumour cells to evade the immune system.
Keyword Biotechnology & Applied Microbiology
Virology
Latent Membrane-protein
Glycosphingolipid-rich Complexes
Transformed-cells
Lymphocytes
Activation
Lysosomes
Membrane-protein-1
Aggregation
Expression
Secretion
Q-Index Code C1

 
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Created: Tue, 14 Aug 2007, 19:38:35 EST